SDS-Page
(SDS-PAGE of 25kDa human Mn SOD (SPR-131).)
SOD (Mn) Recombinant Protein | Sod2 recombinant protein
Recombinant Human Mn SOD
Reactivity
Human
Applications
Western Blot
Synonyms
SOD (Mn); Recombinant Human Mn SOD; Manganese SOD; IPO B; Mn SOD; SOD2; Sod2 recombinant protein
Host
Human
Reactivity
Human
Form/Format
Affinity Purified using His tag
Concentration
0.5mg/mL (varies by lot)
Sequence Length
2090
Applicable Applications for Sod2 recombinant protein
Western Blot (WB) Control
Certificate of Analysis
This product has been certified >90% pure using SDS-PAGE analysis.
Conjugate Tag
His tag
Expression System
E Coli
Storage Buffer
50mM Tris/HCl pH7.7, 0.15M NaCl, 5mM DTT, 10% glycerol
Preparation and Storage
-20 degree C
SDS-Page
(SDS-PAGE of 25kDa human Mn SOD (SPR-131).)
SDS-Page
(SDS-PAGE of 25kDa human Mn SOD (SPR-131).)
Related Product Information for Sod2 recombinant protein
Background Info: Molecular weight of approximately 25kDa
Scientific Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2" to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(8). SOD3 can also be distinguished by its heparin-binding capacity (1).
Scientific Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2" to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(8). SOD3 can also be distinguished by its heparin-binding capacity (1).
Product Categories/Family for Sod2 recombinant protein
References
1. Adachi T., et al. (1992). Clin. Chim. Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22: 111- 180. 3. FurukawaY., and O'Halloran T. (2006). Antioxidants & Redo Signaling. 8 (No 5): 6. 4. Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe N., et al. (1990) Clinica Chimica Acta. 192: 171- 180. 7. Ojika T., et al. (1991) Acta Histochem Cytochem. 24(50): 489-495. 8. Wispe J.R., et al. (1989) BBA. 994: 30-36.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
25kD
NCBI Official Full Name
Rattus norvegicus superoxide dismutase 2, mitochondrial, mRNA
NCBI Official Synonym Full Names
superoxide dismutase 2, mitochondrial
NCBI Official Symbol
Sod2
NCBI Protein Information
superoxide dismutase [Mn], mitochondrial; superoxide dismutase [Mn], mitochondrial; Superoxide dimutase 2, mitochondrial; mitochondrial superoxide dismutase 2
UniProt Protein Name
Superoxide dismutase [Mn], mitochondrial
Protein Family
UniProt Gene Name
SOD2
UniProt Entry Name
SODM_HUMAN
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Product Notes
The Sod2 sod2 (Catalog #AAA806956) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Human Mn SOD reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SOD (Mn) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB) Control. Researchers should empirically determine the suitability of the Sod2 sod2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOD (Mn), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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