Western Blot (WB)
((0. 05ug/ml) staining of Human Peripheral Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat LSP1 Polyclonal Antibody | anti-LSP1 antibody
Goat anti-LSP1 Antibody
Gene Names
LSP1; WP34; pp52
Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
LSP1; Polyclonal Antibody; Goat anti-LSP1 Antibody; Goat Anti-LSP1 (aa261-275) Antibody; lymphocyte-specific protein 1; WP34; pp52; 47 kDa actin binding protein; 52 kDa phosphoprotein; F-actin binding and cytoskeleton associated protein; leufactin (leukocyte F-actin binding protein); leukocyte-specific protein 1; lymphocyte-specific ant; 47 kDa actin binding protein antibody; 52 kDa phosphoprotein antibody; F-actin binding and cytoskeleton associated protein antibody; leufactin (leukocyte F-actin binding protein) antibody; leukocyte-specific protein 1 antibody; lymphocyte-specific ant antibody; lymphocyte-specific protein 1 antibody; pp52 antibody; WP34 antibody; LSP1 antibody; anti-LSP1 antibody
Host
Goat
Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RYKFVATGHGKYEKV
Sequence Length
339
Applicable Applications for anti-LSP1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 50kDa band observed in Human Lymph Node, Tonsil and Peripheral Blood Mononucleocytes lysates (calculated MW of 37.2kDa according to NP_002330.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Liu et al, J Exp Med.2005 Feb 7;201(3): 409-18; PMID: 15684321). Recommended concentration: 0. 05-0.2ug/ml.
Western Blot: Approx 50kDa band observed in Human Lymph Node, Tonsil and Peripheral Blood Mononucleocytes lysates (calculated MW of 37.2kDa according to NP_002330.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Liu et al, J Exp Med.2005 Feb 7;201(3): 409-18; PMID: 15684321). Recommended concentration: 0. 05-0.2ug/ml.
Immunogen
Peptide with sequence C-RYKFVATGHGKYEKV, from the C Terminus of the protein sequence according to NP_002330.1; NP_001013271.1.
Epitope
C Terminus
Note
This antibody is expected to recognize both reported isoforms (NP_002330.1; NP_001013271.1). Reported variants represent identical protein: NP_001013273.1, NP_001013271.1, NP_001013272.1
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0. 05ug/ml) staining of Human Peripheral Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 05ug/ml) staining of Human Peripheral Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 05ug/ml) staining of Human Liver lysate (lane 1), Mouse Liver lysate (lane 2) and Rat Liver lysate (lane 3) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 05ug/ml) staining of Human Liver lysate (lane 1), Mouse Liver lysate (lane 2) and Rat Liver lysate (lane 3) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,389 Da
NCBI Official Full Name
lymphocyte-specific protein 1 isoform 1
NCBI Official Synonym Full Names
lymphocyte-specific protein 1
NCBI Official Symbol
LSP1
NCBI Official Synonym Symbols
WP34; pp52
NCBI Protein Information
lymphocyte-specific protein 1
UniProt Protein Name
Lymphocyte-specific protein 1
Protein Family
UniProt Gene Name
LSP1
UniProt Synonym Gene Names
WP34; pp52
UniProt Entry Name
LSP1_HUMAN
NCBI Description
This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
LSP1: May play a role in mediating neutrophil activation and chemotaxis. Binds actin. Activated T-lymphocytes.
Protein type: Actin-binding; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: actin cytoskeleton; membrane; plasma membrane
Molecular Function: actin binding; signal transducer activity
Biological Process: cell motility; cellular defense response; chemotaxis; signal transduction
Research Articles on LSP1
Similar Products
Product Notes
The LSP1 lsp1 (Catalog #AAA422315) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-LSP1 Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's LSP1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 50kDa band observed in Human Lymph Node, Tonsil and Peripheral Blood Mononucleocytes lysates (calculated MW of 37.2kDa according to NP_002330.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Liu et al, J Exp Med.2005 Feb 7;201(3): 409-18; PMID: 15684321). Recommended concentration: 0. 05-0.2ug/ml. Researchers should empirically determine the suitability of the LSP1 lsp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RYKFVATGHG KYEKV. It is sometimes possible for the material contained within the vial of "LSP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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