Rabbit TRAF6 Antibody | anti-TRAF6 antibody

TRAF6 Antibody

Cat. Num. AAA8502526

• Gene Names: TRAF6; RNF85; MGC:3310
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: TRAF6; TRAF6 Antibody; MGC:3310; RNF85; anti-TRAF6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 522

• Applicable Applications for anti-TRAF6 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human TRAF6
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TRAF6 antibody.)

Related Product Information for anti-TRAF6 antibody

TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses. TRAF6 plays a critical role in innate and adaptive immunity, bone metabolism, and development of certain tissues including the nervous system (5).TRAF6 deficiency results in osteopetrosis and defective IL-1, CD40, and LPS signaling (6) as well as defects in neuronal development (7). Unlike other TRAF family members that mediate signaling through TNF, TRAF6 has unique binding activities (8) that result in signaling responses from the interleukin-1 receptor (IL-1R) (9), toll-like receptor (10, 11), CD40 (12), RANK (13, 14), and p75 neurotrophin receptor (15). TRAF6 associates directly with CD40 and RANK, and indirectly with IL-1R/TLR through IRAK (10). This leads to activation of NF-kappaB and MAP kinase signaling pathways through downstream association with the TAB/TAK-1 complex (16). TRAF6 also activates Src family nonreceptor tyrosine kinases leading to Akt activation (17).

Product Categories/Family for anti-TRAF6 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Arch, R.H. et al. (1998) Genes Dev. 12, 2821-2830. 2. Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-688. 3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-6491. 4. Rothe, M. et al. (1994) Cell 78, 681-692. 5. Wu, H. and Arron, J.R. (2003) Bioessays 25, 1096-105. 6. Lomaga, M.A. et al. (1999) Genes Dev 13, 1015-24. 7. Lomaga, M.A. et al. (2000) J Neurosci 20, 7384-93. 8. Ye, H. et al. (2002) Nature 418, 443-7. 9. Cao, Z. et al. (1996) Nature 383, 443-6. 10. Muzio, M. et al. (1997) Science 278, 1612-5. 11. Medzhitov, R. et al. (1998) Mol Cell 2, 253-8. 12. Ishida, T. et al. (1996) J Biol Chem 271, 28745-8. 13. Darnay, B.G. et al. (1998) J Biol Chem 273, 20551-5. 14. Wong, B.R. et al. (1998) J Biol Chem 273, 28355-9. 15. Khursigara, G. et al. (1999) J Biol Chem 274, 2597-600. 16. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6. 17. Wong, B.R. et al. (1999) Mol Cell 4, 1041-9.

NCBI and Uniprot Product Information

• NCBI GI #: 4759254
• NCBI GeneID: 7189
• NCBI Accession #: NP_004611.1
• NCBI GenBank Nucleotide #: NM_004620.3
• UniProt Accession #: Q9Y4K3; Q8NEH5; A6NKI7; A8KAB3; D3DR16
• Molecular Weight: 60kDa
• NCBI Official Full Name: TNF receptor-associated factor 6
• NCBI Official Synonym Full Names: TNF receptor associated factor 6
• NCBI Official Symbol: TRAF6
• NCBI Official Synonym Symbols: RNF85; MGC:3310
• NCBI Protein Information: TNF receptor-associated factor 6
• UniProt Protein Name: TNF receptor-associated factor 6
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: TRAF6
• UniProt Synonym Gene Names: RNF85

NCBI Description

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. This protein mediates signaling from members of the TNF receptor superfamily as well as the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. This protein also interacts with the transforming growth factor (TGF) beta receptor complex and is required for Smad-independent activation of the JNK and p38 kinases. This protein has an amino terminal RING domain which is followed by four zinc-finger motifs, a central coiled-coil region and a highly conserved carboxyl terminal domain, known as the TRAF-C domain. Two alternatively spliced transcript variants, encoding an identical protein, have been reported. [provided by RefSeq, Feb 2012]

Uniprot Description

E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production.