Goat SNCA / Alpha-Synuclein Polyclonal Antibody | anti-SNCA antibody

Goat Polyclonal to Human SNCA / Alpha-Synuclein

Cat. Num. AAA248938

• Gene Names: SNCA; PD1; NACP; PARK1; PARK4
• Reactivity: Human, Monkey
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: SNCA / Alpha-Synuclein; Polyclonal Antibody; Goat Polyclonal to Human SNCA / Alpha-Synuclein; Anti-SNCA / Alpha-Synuclein Antibody (Internal) IHC-plus; SNCA; Alpha-synuclein; PARK4; PD1; Synuclein alpha-140; NACP; PARK1; Human SNCA; Alpha-Synuclein; anti-SNCA antibody

• Host: Goat
• Reactivity: Human, Monkey
• Clonality: Polyclonal
• Specificity: Human SNCA / Alpha-Synuclein. This antibody is expected to recognize reported isoform NACP140 (NP_000336.1) only. Reported variants represent identical protein: NP_000336.1, NP_001139527.1, NP_001139526.1
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 140

• Applicable Applications for anti-SNCA antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Peptide Enzyme-Linked Immunoabsorbent Assay
• Application Notes: IHC-P (5 ug/ml); WB (0.1 - 0.3 ug/ml); Peptide Enzyme-Linked Immunoabsorbent Assay : (1:32000); Optimal dilution should be determined by the researcher.
• Target: SNCA / Alpha-Synuclein
• Immunogen: SNCA / Alpha-Synuclein antibody was raised against peptide with sequence C-KDQLGKNEEGAPQE, from the internal region of the protein sequence according to NP_000336.1
• Epitope: aa97-110
• Immunogen species: SNCA / ALPHA-Synucein antibody was raised against Human.
• Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 17kD band observed in Human Brain (Amygdala) lysates (calculated MW of 14.5kD according to NP_000336.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 ug/ml.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-SNCA / Alpha-Synuclein antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(SNCA antibody (0.1 ug/ml) staining of Human Amygdala lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4507109
• NCBI GeneID: 6622
• NCBI Accession #: NP_000336.1
• NCBI GenBank Nucleotide #: NM_000345.3
• UniProt Accession #: P37840; Q13701; Q4JHI3; Q6IAU6; A8K2A4
• NCBI Official Full Name: alpha-synuclein isoform NACP140
• NCBI Official Synonym Full Names: synuclein, alpha (non A4 component of amyloid precursor)
• NCBI Official Symbol: SNCA
• NCBI Official Synonym Symbols: PD1; NACP; PARK1; PARK4
• NCBI Protein Information: alpha-synuclein; synuclein alpha-140; non A-beta component of AD amyloid
• UniProt Protein Name: Alpha-synuclein
• Protein Family: Alpha-synuclein
• UniProt Gene Name: SNCA
• UniProt Synonym Gene Names: NACP; PARK1; NACP
• UniProt Entry Name: SYUA_HUMAN

NCBI Description

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

SNCA: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May integrate presynaptic signaling and membrane trafficking. Implicated in the pathogenesis of Parkinson's disease. A major component of amyloid plaques in the brains of patients with Alzheimer's disease. Two alternatively spliced isoforms transcripts have been identified.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: Golgi apparatus; nuclear outer membrane; mitochondrion; rough endoplasmic reticulum; lysosome; fibril; extracellular region; terminal button; cell cortex; inclusion body; mitochondrial respiratory chain complex I; cytosol; actin cytoskeleton; synaptic vesicle; platelet alpha granule membrane; growth cone; axon; perinuclear region of cytoplasm; cytoplasm; plasma membrane; ribosome; nucleus; cell junction

Molecular Function: protein domain specific binding; identical protein binding; zinc ion binding; histone binding; kinesin binding; ferrous iron binding; microtubule binding; caspase inhibitor activity; beta-tubulin binding; magnesium ion binding; protein N-terminus binding; phosphoprotein binding; oxidoreductase activity; Hsp70 protein binding; calcium ion binding; dynein binding; protein binding; phospholipase binding; copper ion binding; phospholipid binding; tau protein binding; fatty acid binding; alpha-tubulin binding

Biological Process: regulation of acyl-CoA biosynthetic process; adult locomotory behavior; positive regulation of apoptosis; positive regulation of endocytosis; response to lipopolysaccharide; dopamine biosynthetic process; positive regulation of neurotransmitter secretion; calcium ion homeostasis; response to magnesium ion; fibril organization and biogenesis; synapse organization and biogenesis; dopamine uptake; negative regulation of neuron apoptosis; response to drug; negative regulation of dopamine metabolic process; synaptic vesicle endocytosis; negative regulation of transporter activity; negative regulation of apoptosis; regulation of long-term neuronal synaptic plasticity; negative regulation of serotonin uptake; negative regulation of norepinephrine uptake; mitochondrial membrane organization and biogenesis; microglial cell activation; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of monooxygenase activity; fatty acid metabolic process; regulation of dopamine secretion; negative regulation of histone acetylation; negative regulation of dopamine uptake; negative regulation of exocytosis; negative regulation of protein amino acid phosphorylation; behavioral response to cocaine; receptor internalization; phospholipid metabolic process; response to iron(II) ion; positive regulation of receptor recycling; aging; caspase activation; neutral lipid metabolic process; protein destabilization; negative regulation of microtubule polymerization; regulation of glutamate secretion; regulation of macrophage activation; positive regulation of peptidyl-serine phosphorylation; organelle ATP synthesis coupled electron transport; regulation of locomotion; positive regulation of release of sequestered calcium ion into cytosol; regulation of excitatory postsynaptic membrane potential

Disease: Parkinson Disease 4, Autosomal Dominant; Parkinson Disease 1, Autosomal Dominant; Dementia, Lewy Body