Rabbit NID1 / Entactin / Nidogen-1 Polyclonal Antibody | anti-NID1 antibody

NID1, Enactin, Nidogen 1, NID, Nidogen, Entactin, NID-1, Nidogen (enactin), Nidogen-1

Cat. Num. AAA2400150

• Gene Names: NID1; NID
• Reactivity: Mouse, Rat, Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity purified
• Synonyms: NID1 / Entactin / Nidogen-1; Polyclonal Antibody; NID1; Enactin; Nidogen 1; NID; Nidogen; Entactin; NID-1; Nidogen (enactin); Nidogen-1; Rabbit Polyclonal (IgG) to Human NID1 / Entactin / Nidogen-1; Human NID1 / Entactin / Nidogen-1; anti-NID1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NID1 Antibody detects endogenous levels of total NID1 protein.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1247

• Applicable Applications for anti-NID1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:20000); IHC-Paraffin (1:50); WB (1:500 - 1:1000); ; Optimal dilution to be determined by researcher
• Immunogen: NID1 / Entactin / Nidogen-1 antibody was raised against synthetic peptide from human NID1 (aa219-268).
• Immunogen Species: NID1 / Entactin / Nidogen-1 antibody was raised against Human
• Epitope: aa219-268
• Target: NID1/ Entactin/ Nidogen-1
• Antigen Type: Synthetic Peptide
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-NID1 / Entactin / Nidogen antibody IHC staining of human placenta, endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(Western blot of extracts from K562 cells, using NID1 Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-NID1 antibody

NID1 Antibody, Enactin Antibody, Nidogen 1 Antibody, NID Antibody, Nidogen Antibody, Entactin Antibody, NID-1 Antibody, Nidogen (enactin) Antibody, Nidogen-1 Antibody Description: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

NCBI and Uniprot Product Information

• NCBI GI #: 115298674
• NCBI GeneID: 4811
• NCBI Accession #: NP_002499.2
• NCBI GenBank Nucleotide #: NM_002508.2
• UniProt Accession #: P14543; Q14942; Q59FL2; Q5TAF2; Q5TAF3; Q86XD7
• Molecular Weight: 122,017 Da
• NCBI Official Full Name: nidogen-1
• NCBI Official Synonym Full Names: nidogen 1
• NCBI Official Symbol: NID1
• NCBI Official Synonym Symbols: NID
• NCBI Protein Information: nidogen-1
• UniProt Protein Name: Nidogen-1
• Protein Family: Nidogen
• UniProt Gene Name: NID1
• UniProt Synonym Gene Names: NID; NID-1
• UniProt Entry Name: NID1_HUMAN

NCBI Description

This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008]

Uniprot Description

Nidogen-1: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: basal lamina; basement membrane; extracellular matrix; extracellular region

Molecular Function: calcium ion binding; collagen binding; laminin binding; laminin-1 binding; proteoglycan binding

Biological Process: cell-matrix adhesion; extracellular matrix disassembly; extracellular matrix organization and biogenesis; glomerular basement membrane development