Mouse anti-Human FARSB Monoclonal Antibody | anti-FARSB antibody

Mouse Monoclonal [clone 2F11] (IgG2a,k) to Human FARSB

Cat. Num. AAA246201

• Gene Names: FARSB; FRSB; PheHB; PheRS; FARSLB; HSPC173
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA, Immunohistochemistry
• Purity: Purified from ascites by Protein A
• Synonyms: FARSB; Monoclonal Antibody; Mouse Monoclonal [clone 2F11] (IgG2a; k) to Human FARSB; FARSLB; FRSB; HSPC173; PheHB; PheRS; anti-FARSB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2F11
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.45 mg/ml (varies by lot)
• Sequence Length: 589

• Applicable Applications for anti-FARSB antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: IHC-Paraffin (10ug/ml); (Optimal dilution to be determined by the researcher)
• Target Species: Human
• Immunogen Species: FARSB antibody was raised against Human
• Immunogen Type: Recombinant protein
• Immunogen: FARSB antibody was raised against fARSLB (NP_005678, 234 a.a. ~ 342 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-FARSB antibody IHC of human colon, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-FARSB antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-FARSB antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(FARSLB monoclonal antibody clone 2F11 Western blot of FARSLB expression in HepG2.)

NCBI and Uniprot Product Information

• NCBI GI #: 124028525
• NCBI GeneID: 10056
• NCBI Accession #: NP_005678.3
• NCBI GenBank Nucleotide #: NM_005687.4
• UniProt Accession #: Q9NSD9; O95708; Q4ZFX1; Q57ZJ5; Q9NZZ6; B4DFM0
• Molecular Weight: 54,861 Da
• NCBI Official Full Name: phenylalanine--tRNA ligase beta subunit
• NCBI Official Synonym Full Names: phenylalanyl-tRNA synthetase, beta subunit
• NCBI Official Symbol: FARSB
• NCBI Official Synonym Symbols: FRSB; PheHB; PheRS; FARSLB; HSPC173
• NCBI Protein Information: phenylalanine--tRNA ligase beta subunit; phenylalanine-tRNA ligase beta chain; phenylalanine--tRNA ligase beta chain; phenylalanyl-tRNA synthetase beta chain; phenylalanyl-tRNA synthetase beta-subunit; phenylalanine tRNA ligase 1, beta, cytoplasmic; phenylalanyl-tRNA synthetase-like, beta subunit; phenylalanine-tRNA synthetase-like, beta subunit
• UniProt Protein Name: Phenylalanine--tRNA ligase beta subunit
• UniProt Gene Name: FARSB
• UniProt Synonym Gene Names: FARSLB; FRSB; PheRS
• UniProt Entry Name: SYFB_HUMAN

NCBI Description

This gene encodes a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

FARSLB: a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. [provided by RefSeq, Jul 2008]

Protein type: EC 6.1.1.20; Translation; Ligase

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: RNA binding; phenylalanine-tRNA ligase activity; magnesium ion binding; ATP binding

Biological Process: tRNA aminoacylation for protein translation; translation; phenylalanyl-tRNA aminoacylation; gene expression