Western Blot/Blotting
(Western blot using nSMase2 antibody on human brain lysate . Lysate used at 14 ug/lane. Antibody used at 10)
Rabbit anti-Human Sphingomyelin Phosphodiesterase 3 Polyclonal Antibody | anti-COL4A3BP antibody
Sphingomyelin Phosphodiesterase 3
Western Blot/Blotting
(Western blot using nSMase2 antibody on human brain lysate . Lysate used at 14 ug/lane. Antibody used at 10)
Western Blot/Blotting
(Western blot using nSMase2 antibody on human brain lysate . Lysate used at 14 ug/lane. Antibody used at 10)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner. Ref.3 Ref.11 Ref.16 Ref.17
Subunit structure: Interacts with COL4A3. Interacts with VAPA and VAPB. Interaction with VAPB is less efficient than with VAPA. Ref.10
Subcellular location: Cytoplasm. Golgi apparatus. Endoplasmic reticulum. Note: Preferentially localized to the Golgi apparatus. Ref.3 Ref.10 Ref.16
Tissue specificity: Widely expressed.
Domain: The START domain recognizes ceramides and diacylglycerol lipids, interacts with membranes, and mediates the intermembrane transfer of ceramides and diacylglycerol lipids. Ref.3The PH domain targets the Golgi apparatus. Ref.3The FFAT motif is required for interaction with VAPA and VAPB. Ref.3
Post-translational modification: Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity. Inactivated by hyperphosphorylation of serine residues by CSNK1G2/CK1 that triggers dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis.
Sequence similarities: Contains 1 PH domain.Contains 1 START domain.
Caution: Was originally (Ref.1) reported to have a protein kinase activity and to phosphorylate on Ser and Thr residues the Goodpasture autoantigen (in vitro).
Research Articles on COL4A3BP
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Product Notes
The COL4A3BP col4a3bp (Catalog #AAA395854) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Sphingomyelin Phosphodiesterase 3 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Sphingomyelin Phosphodiesterase 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the COL4A3BP col4a3bp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sphingomyelin Phosphodiesterase 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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