Rabbit anti-Human, Mouse SKA2 Polyclonal Antibody | anti-SKA2 antibody

SKA2 Antibody

Cat. Num. AAA150123

• Gene Names: SKA2; FAM33A
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: SKA2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: SKA2; Polyclonal Antibody; SKA2 Antibody; FAM33A; Spindle and kinetochore-associated protein 2; Protein FAM33A; spindle and kinetochore associated complex subunit 2; anti-SKA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: SKA2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 121

• Applicable Applications for anti-SKA2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: SKA2 antibody can be used for detection of SKA2 by Western blot at 0.5 - 1 mug/mL.
• Conjugate: Unconjugated
• Immunogen: SKA2 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human SKA2.
• Buffer: SKA2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SKA2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SKA2 in 3T3 cell lysate with SKA2 antibody at (A) 0.5 and (B) 1 μg/mL.)

Related Product Information for anti-SKA2 antibody

SKA2 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing. SKA2 has also been shown to interact with glucocorticoid receptors and to be involved in glucocorticoid signaling and cell proliferation.

NCBI and Uniprot Product Information

• NCBI GI #: 32699054
• NCBI GeneID: 348235
• NCBI Accession #: NP_872426
• NCBI GenBank Nucleotide #: NM_182620.3
• UniProt Accession #: Q8WVK7; A6NIL3; B3KPL3; E9PCB8
• Molecular Weight: 8,282 Da
• NCBI Official Full Name: spindle and kinetochore-associated protein 2 isoform 1
• NCBI Official Synonym Full Names: spindle and kinetochore associated complex subunit 2
• NCBI Official Symbol: SKA2
• NCBI Official Synonym Symbols: FAM33A
• NCBI Protein Information: spindle and kinetochore-associated protein 2; spindle and KT (kinetochore) associated 2; family with sequence similarity 33, member A
• UniProt Protein Name: Spindle and kinetochore-associated protein 2
• Protein Family: Spindle and kinetochore-associated protein
• UniProt Gene Name: SKA2
• UniProt Synonym Gene Names: FAM33A
• UniProt Entry Name: SKA2_HUMAN

Uniprot Description

SKA2: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization. Belongs to the SKA2 family.

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: spindle microtubule; outer kinetochore of condensed chromosome; cytosol

Molecular Function: protein binding; microtubule binding

Biological Process: mitosis; cell division; mitotic cell cycle; regulation of microtubule polymerization or depolymerization; chromosome segregation