Western Blot (WB)
(SKA2 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the SKA2 antibody detected the SKA2 protein (arrow).)
Rabbit anti-Human SKA2 Polyclonal Antibody | anti-SKA2 antibody
SKA2, NT (SKA2, FAM33A, Spindle and kinetochore-associated protein 2, Protein FAM33A)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Immunohistochemistry (FFPE): 1:10 - 1:50
Optimal dilutions to be determined by the researcher.
Species Sequence Homology: monkey
Western Blot (WB)
(SKA2 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the SKA2 antibody detected the SKA2 protein (arrow).)
Western Blot (WB)
(SKA2 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the SKA2 antibody detected the SKA2 protein (arrow).)
Immunohistochemistry (IHC)
(SKA2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SKA2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SKA2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SKA2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
Uniprot Description
SKA2: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization. Belongs to the SKA2 family.
Chromosomal Location of Human Ortholog: 17q22
Cellular Component: spindle microtubule; outer kinetochore of condensed chromosome; cytosol
Molecular Function: protein binding; microtubule binding
Biological Process: mitosis; cell division; mitotic cell cycle; regulation of microtubule polymerization or depolymerization; chromosome segregation
Research Articles on SKA2
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Product Notes
The SKA2 ska2 (Catalog #AAA6010651) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SKA2, NT (SKA2, FAM33A, Spindle and kinetochore-associated protein 2, Protein FAM33A) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SKA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000 Immunohistochemistry (FFPE): 1:10 - 1:50 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the SKA2 ska2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SKA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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