GDNF active protein

Human GDNF Protein

Cat. Num. AAA9718683

• Gene Names: GDNF; ATF; ATF1; ATF2; HSCR3; HFB1-GDNF
• Purity: >97% as determined by SDS-PAGE
• Synonyms: GDNF; Human GDNF Protein; Recombinant Human GDNF Protein (Glial cell line-derived neurotrophic factor) expressed in Human Cells; ATF1; ATF2; HFB1-GDNF; HSCR3; Glial cell line-derived neurotrophic factor; GDNF active protein

• Host: Human Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence: Amino acid sequence derived from mature form of human GDNF (P39905-2) (Arg 83-Ile 185) was expressed and purified, with additional two aa (Gly& Pro) at the N-terminus.
• Sequence Length: 159

• Species: Human
• Endotoxin: The Endotoxin level is less than 1.0 EU per mug of the protein as determined by the LAL method
• Protein Length: The recombinant human GDNF consists of 105 amino acids and predicts a molecular mass of 15.1 KDa. It migrates as approximately 18 KDa band in SDS-PAGE under reducing conditions.
• Activity: 1. Measured in a cell proliferation assay using SH?SY5Y human neuroblastoma cells. The ED50 for this effect is typically 3-15 ng/mL in the presence of Recombinant Human GFR alpha?1/GDNF R alpha?1 His Chimera. 2. Measured by its ability to bind biotinylated Human GFRa1-His in functional ELISA.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human GDNF protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human GDNF protein.)

Related Product Information for GDNF active protein

Glial cell line-derived neurotrophic factor(GDNF) is an important member of the GDNF family of ligands(GFL). The GDNF family of ligands is comprised by four neurotrophic factors: glial cell line-derived neurotrophic factor (GDNF), neurturin (NRTN), artemin (ARTN), and persephin (PSPN). It has been found that GFLs play a role in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration. As the founding member, GDNF plays a key role in the promotion of the survival of dopaminergic neurons. GDNF is a highly conserved neurotrophic factor. The recombinant form of this protein also promotes the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. GDNF also regulates kidney development and spermatogenesis, and it affects alcohol consumption. It has been shown that GDNF results in two Parkinson's disease clinical trial and in a number of animal trials. It has been taken as a potent survival factor for central motoneurons.

NCBI and Uniprot Product Information

• NCBI GI #: 493796159
• NCBI GeneID: 2668
• NCBI Accession #: NP_001265027.1
• NCBI GenBank Nucleotide #: NP_001265027.1
• UniProt Accession #: P39905
• Molecular Weight: 15.1 kDa
• NCBI Official Full Name: glial cell line-derived neurotrophic factor isoform 5
• NCBI Official Synonym Full Names: glial cell derived neurotrophic factor
• NCBI Official Symbol: GDNF
• NCBI Official Synonym Symbols: ATF; ATF1; ATF2; HSCR3; HFB1-GDNF
• NCBI Protein Information: glial cell line-derived neurotrophic factor
• UniProt Protein Name: Glial cell line-derived neurotrophic factor
• Protein Family: GDNF family receptor
• UniProt Gene Name: GDNF
• UniProt Synonym Gene Names: hGDNF; ATF
• UniProt Entry Name: GDNF_HUMAN

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Mutations in this gene may be associated with Hirschsprung disease and Tourette syndrome. This gene encodes multiple protein isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016]

Uniprot Description

GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5p13.1-p12

Cellular Component: extracellular region

Molecular Function: protein homodimerization activity; growth factor activity; receptor binding

Biological Process: nervous system development; axon guidance; positive regulation of dopamine secretion; peristalsis; adult locomotory behavior; mRNA stabilization; positive regulation of monooxygenase activity; regulation of dopamine uptake; signal transduction; enteric nervous system development; sympathetic nervous system development; regulation of gene expression; ureteric bud branching; induction of an organ; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; postganglionic parasympathetic nervous system development; negative regulation of neuron apoptosis; postsynaptic membrane organization; positive regulation of cell differentiation; neural crest cell migration; metanephros development; neurite development; negative regulation of apoptosis

Disease: Hirschsprung Disease, Susceptibility To, 3; Central Hypoventilation Syndrome, Congenital; Pheochromocytoma