Porcine Platelet Factor 4 (PF4) ELISA Kit | PF4 elisa kit

Pig Platelet Factor 4 (PF4) ELISA Kit

Cat. Num. AAA2701434

• Gene Names: PF4; PF-4; CXCL4; SCYB4
• Reactivity: Porcine
• Synonyms: Platelet Factor 4 (PF4); Pig Platelet Factor 4 (PF4) ELISA Kit; CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact; PF4 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of PF4. No significant cross-reactivity or interference between PF4 and analogues was observed.
• Sequence Length: 101

• Samples: Serum, platelet-poor plasma and cell culture supernates
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1,000ng/mL
• Sensitivity: < 5.7ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PF4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PF4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for PF4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PF4 in porcine serum, platelet-poor plasma and cell culture supernates.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PF4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PF4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505733
• NCBI GeneID: 5196
• NCBI Accession #: NP_002610.1
• NCBI GenBank Nucleotide #: NM_002619.3
• UniProt Accession #: P02776; Q53X61; Q9UC64; Q9UC65
• Molecular Weight: 10,845 Da
• NCBI Official Full Name: platelet factor 4
• NCBI Official Synonym Full Names: platelet factor 4
• NCBI Official Symbol: PF4
• NCBI Official Synonym Symbols: PF-4; CXCL4; SCYB4
• NCBI Protein Information: platelet factor 4
• UniProt Protein Name: Platelet factor 4
• Protein Family: Platelet factor
• UniProt Gene Name: PF4
• UniProt Synonym Gene Names: CXCL4; SCYB4; PF-4

NCBI Description

This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. The protein also exhibits antimicrobial activity against Plasmodium falciparum. [provided by RefSeq, Oct 2014]

Uniprot Description

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.