Rabbit anti-Human, Mouse SIRT6 Polyclonal Antibody | anti-SIRT6 antibody

SIRT6, CT (NAD-dependent Deacetylase Sirtuin-6, SIR2-like Protein 6, SIR2L6)

Cat. Num. AAA625195

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: SIRT6; Polyclonal Antibody; CT (NAD-dependent Deacetylase Sirtuin-6; SIR2-like Protein 6; SIR2L6); Anti -SIRT6; anti-SIRT6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Sirt6. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-SIRT6 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human Sirt6 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of SIRT6 Antibody (C-term) in mouse stomach tissue lysates (35ug/lane). SIRT6 (arrow) was detected using the purified Pab.)

Related Product Information for anti-SIRT6 antibody

SIRT6 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.

Product Categories/Family for anti-SIRT6 antibody

Antibodies; Abs to Sirtuins

NCBI and Uniprot Product Information

• NCBI GI #: 48146517
• UniProt Accession #: Q8N6T7; O75291; Q6IAF5; Q6PK99; Q8NCD2; Q9BSI5; Q9BWP3; Q9NRC7; Q9UQD1; B2RCD0
• Molecular Weight: 39,119 Da
• NCBI Official Full Name: SIRT6
• UniProt Protein Name: NAD-dependent deacetylase sirtuin-6
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: SIRT6
• UniProt Synonym Gene Names: SIR2L6
• UniProt Entry Name: SIRT6_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010]

Uniprot Description

SIRT6: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 3.5.1.-; Deacetylase; EC 2.4.2.31

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleolus; nuclear telomeric heterochromatin; nucleus

Molecular Function: NAD(P)+-protein-arginine ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; zinc ion binding; NAD-dependent histone deacetylase activity; chromatin binding; NAD+ ADP-ribosyltransferase activity

Biological Process: protein amino acid ADP-ribosylation; histone deacetylation