Growth Differentiation Factor 7 Recombinant Protein | GDF7 recombinant protein

Recombinant Growth Differentiation Factor 7 (GDF7)

Cat. Num. AAA2031027

• Gene Names: GDF7; BMP12
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Growth Differentiation Factor 7; Recombinant Growth Differentiation Factor 7 (GDF7); GDF7 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 9.5 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SRKANL GGRRRRRTAL AGTRGAQGSG GGGGGGGGGG GGGGGGGGGA GRGHGRRGRS RCSRKSLHVD FKELGWDDWI IAPLDYEAYH CEGVCDFPLR SHLEPTNHAI IQTLLNSMAP DAAPASCCVPARLSPISILY IDAANNVVYK QYEDMVV
• Sequence Length: 453

• Applicable Applications for GDF7 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 19.7kDa
• Accurate Molecular Mass (KD): 17kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Ser295~Val447
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 40786371
• NCBI GeneID: 151449
• Molecular Weight: 46,950 Da
• NCBI Official Full Name: growth differentiation factor 7
• NCBI Official Synonym Full Names: growth differentiation factor 7
• NCBI Official Symbol: GDF7
• NCBI Official Synonym Symbols: BMP12
• NCBI Protein Information: growth/differentiation factor 7
• UniProt Protein Name: Growth/differentiation factor 7
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF7
• UniProt Synonym Gene Names: GDF-7
• UniProt Entry Name: GDF7_HUMAN

NCBI Description

This gene encodes a member of the bone morphogenetic protein (BMP) family. BMPs belong to the transforming growth factor-beta superfamily of secreted signalling molecules that regulate diverse processes in growth, repair and embryonic development. In mouse, this gene functions as an inductive signal from the roof plate required for the specification of neuronal identity in the dorsal spinal cord. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF7: May play an active role in the motor area of the primate neocortex. Belongs to the TGF-beta family.

Protein type: Cytokine; Secreted, signal peptide; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 2p24.1

Cellular Component: extracellular space

Molecular Function: protein binding; protein homodimerization activity; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: axon guidance; cell fate commitment; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; roof plate formation; forebrain morphogenesis; BMP signaling pathway; regulation of apoptosis; branching morphogenesis of a tube; epithelial cell differentiation; regulation of MAPKKK cascade; midbrain development; spinal cord association neuron differentiation; gland morphogenesis; positive regulation of neuron differentiation; reproductive structure development; cell development; growth