DCTN2 recombinant protein

DCTN2, 1-406aa, Human, His tag, E Coli

Cat. Num. AAA204975

• Gene Names: DCTN2; RBP50; DCTN50; HEL-S-77; DYNAMITIN
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: DCTN2; 1-406aa; Human; His tag; E Coli; Dynactin subunit 2 isoform 1; Dynactin 2 (p50); DCTN50; DYNAMITIN; RBP50; DCTN2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl (pH 8.0) containing 20% glycerol, 0.15M NaCl, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADPKYA DLPGIARNEP DVYETSDLPE DDQAEFDAFA QELEELTSTS VEHIIVNPNA AYDKFKDKRV GTKGLDFSDR IGKTKRTGYE SGEYEMLGEG LGVKETPQQK YQRLLHEVQE LTTEVEKIKT TVKESATEEK LTPVLLAKQL AALKQQLVAS HLEKLLGPDA AINLTDPDGA LAKRLLLQLE ATKNSKGGSG GKTTGTPPDS SLVTYELHSR PEQDKFSQAA KVAELEKRLT ELETAVRCDQ DAQNPLSAGL QGACLMETVE LLQAKVSALD LAVLDQVEAR LQSVLGKVNE IAKHKASVED ADTQSKVHQL YETIQRWSPI ASTLPELVQR LVTIKQLHEQ AMQFGQLLTH LDTTQQMIAN SLKDNTTLLT QVQTTMRENL ATVEGNFASI DERMKKLGK
• Sequence Length: 406

• Applicable Applications for DCTN2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for DCTN2 recombinant protein

DCTN2 is a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Recombinant human DCTN2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for DCTN2 recombinant protein

Signal Transduction

References

Echeverri C.J., Paschal B.M. et al. (1996), J. Cell Biol. 132:617-633

NCBI and Uniprot Product Information

• NCBI GI #: 5453629
• NCBI GeneID: 10540
• NCBI Accession #: NP_006391
• NCBI GenBank Nucleotide #: NM_006400.4
• UniProt Accession #: Q13561; Q86YN2; Q9BW17; B2RBK5
• Molecular Weight: 47.2kDa (429aa) confirmed by MALDI-TOF
• NCBI Official Full Name: dynactin subunit 2 isoform 1
• NCBI Official Synonym Full Names: dynactin subunit 2
• NCBI Official Symbol: DCTN2
• NCBI Official Synonym Symbols: RBP50; DCTN50; HEL-S-77; DYNAMITIN
• NCBI Protein Information: dynactin subunit 2
• UniProt Protein Name: Dynactin subunit 2
• Protein Family: Dynactin
• UniProt Gene Name: DCTN2
• UniProt Synonym Gene Names: DCTN50; DCTN-50

NCBI Description

This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.