Western Blot (WB)
(Western Blot: Sample: Human Hela Cells.)
Rabbit anti-Human TTK Protein Kinase Polyclonal Antibody | anti-TTK antibody
Polyclonal Antibody to TTK Protein Kinase (TTK)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GSGGSSKVF QVLNEKKQIY AIKYVNLEEA DNQTLDSYRN EIAYLNKLQQ HSDKIIRLYD YEITDQYIYM VMECGNIDLN SWLKKKKSID PWERKSYWKN MLEAVHTIHQ HGIVHSDLKP ANFLIVDGML KLIDFGIANQ MQPDTTSVVK DSQVGTVNYM PPEAIKDMSS SRENGKSKSK ISPKSDVWSL GCILYYMTYG
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant TTK, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant TTK, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples:Human Skin Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples:Human Skin Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples:Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples:Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(FITC staining on IHC-P Simple: Mcf7 cells))
Immunohistochemistry (IHC)
(FITC staining on IHC-P Simple: Mcf7 cells))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples:Human Liver Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples:Human Liver Cancer Tissue)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: TTK knockout Hela cell lysate; Predicted MW: 97kd Observed MW: 100kd Primary Ab: 5ug/ml Rabbit Anti-Human TTK Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: TTK knockout Hela cell lysate; Predicted MW: 97kd Observed MW: 100kd Primary Ab: 5ug/ml Rabbit Anti-Human TTK Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]
Uniprot Description
TTK: a dual-specificity protein kinase orthologous to the yeast monopolar spindle (Mps1) kinase that is required for spindle pole duplication and the spindle checkpoint but not for centrosome duplication. Localized to centrosomes, kinetochores and nuclear pores. Its enzymatic activity is required for the recruitment of Mad1, Mad2, Bub1 and Bub3 to the kinetochore. Mps1 mRNA levels change little throughout the cell cycle, but its protein expression varies during the cell cycle. Overexpression arrests cells in metaphase by activating the checkpoint.
Protein type: Cancer Testis Antigen (CTA); EC 2.7.12.1; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, dual-specificity (non-receptor); TTK family
Chromosomal Location of Human Ortholog: 6q14.1
Cellular Component: cytoplasm; kinetochore; membrane; spindle
Molecular Function: ATP binding; protein binding; protein homodimerization activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein tyrosine kinase activity
Biological Process: chromosome separation; female meiosis chromosome segregation; meiotic spindle assembly checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic spindle organization and biogenesis; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; peptidyl-tyrosine phosphorylation; positive regulation of cell proliferation; positive regulation of pathway-restricted SMAD protein phosphorylation; protein autophosphorylation; protein localization to kinetochore; spindle organization and biogenesis
Research Articles on TTK
Similar Products
Product Notes
The TTK ttk (Catalog #AAA2027392) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to TTK Protein Kinase (TTK) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TTK Protein Kinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1-5ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the TTK ttk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GSGGSSKVF QVLNEKKQIY AIKYVNLEEA DNQTLDSYRN EIAYLNKLQQ HSDKIIRLYD YEITDQYIYM VMECGNIDLN SWLKKKKSID PWERKSYWKN MLEAVHTIHQ HGIVHSDLKP ANFLIVDGML KLIDFGIANQ MQPDTTSVVK DSQVGTVNYM PPEAIKDMSS SRENGKSKSK ISPKSDVWSL GCILYYMTYG. It is sometimes possible for the material contained within the vial of "TTK Protein Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
