Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Septin-12 (SEPT12) Standard Curve.)
Human Septin-12 (SEPT12) ELISA Kit | SEPT12 elisa kit
Human Septin-12 (SEPT12) ELISA Kit
Gene Names
SEPTIN12; SEPT12; SPGF10
Reactivity
Human
Synonyms
Septin-12 (SEPT12); Human Septin-12 (SEPT12) ELISA Kit; SEPT12; FLJ25410; SEPT12 elisa kit
Reactivity
Human
Specificity
Human Septin-12 (SEPT12) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEPT12. No significant cross-reactivity or interference between Human SEPT12 and analogues was observed.
Sequence Length
312
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Septin-12 (SEPT12) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Septin-12 (SEPT12) Standard Curve.)
Related Product Information for SEPT12 elisa kit
Description: This Human Septin-12 (SEPT12) ELISA Kit employs a two-site sandwich ELISA to quantitate SEPT12 in samples. An antibody specific for SEPT12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEPT12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEPT12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEPT12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Septins are a group of highly conserved GTP binding proteins found in eukaryotes. In yeast cells, they build scaffolding to provide structural support during cell division and compartmentalize parts of the cell. Septins, such as SEPT12, are conserved GTP-binding proteins that function as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeleton remodeling, as well as infection, neurodegeneration, and neoplasia. By searching databases for members of the septin family, Hall et al. (2005) identified SEPT12. DNA microarray analysis showed low expression of SEPT12 in all normal, diseased, and cancer tissues examined.By genomic sequence analysis, Hall et al. (2005) mapped the SEPT12 gene to chromosome 16p13.3.
Background: Septins are a group of highly conserved GTP binding proteins found in eukaryotes. In yeast cells, they build scaffolding to provide structural support during cell division and compartmentalize parts of the cell. Septins, such as SEPT12, are conserved GTP-binding proteins that function as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeleton remodeling, as well as infection, neurodegeneration, and neoplasia. By searching databases for members of the septin family, Hall et al. (2005) identified SEPT12. DNA microarray analysis showed low expression of SEPT12 in all normal, diseased, and cancer tissues examined.By genomic sequence analysis, Hall et al. (2005) mapped the SEPT12 gene to chromosome 16p13.3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
septin-12 isoform 1
NCBI Official Synonym Full Names
septin 12
NCBI Official Symbol
SEPTIN12
NCBI Official Synonym Symbols
SEPT12; SPGF10
NCBI Protein Information
septin-12
UniProt Protein Name
Septin-12
UniProt Gene Name
SEPT12
UniProt Entry Name
SEP12_HUMAN
NCBI Description
This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Research Articles on SEPT12
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Product Notes
The Human SEPT12 sept12 (Catalog #AAA9714493) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714493 ELISA Kit recognizes Human SEPT12. It is sometimes possible for the material contained within the vial of "Septin-12 (SEPT12), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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