Western Blot (WB)
(Western Blot analysis of SEPT12 expression in transfected 293T cell line by SEPT12 polyclonal antibody. Lane 1: SEPT12 transfected lysate (39.38kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human SEPT12 Polyclonal Antibody | anti-SEPT12 antibody
SEPT12 (Septin 12, FLJ25410, Septin-12)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Western Blot (WB)
(Western Blot analysis of SEPT12 expression in transfected 293T cell line by SEPT12 polyclonal antibody. Lane 1: SEPT12 transfected lysate (39.38kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of SEPT12 expression in transfected 293T cell line by SEPT12 polyclonal antibody. Lane 1: SEPT12 transfected lysate (39.38kD). Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC)
(Immunoperoxidase of purified antibody to SEPT12 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
Immunohistochemistry (IHC)
(Immunoperoxidase of purified antibody to SEPT12 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to SEPT12 on HeLa cell. [antibody concentration 10ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to SEPT12 on HeLa cell. [antibody concentration 10ug/ml].)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
Function: Filament-forming cytoskeletal GTPase
By similarity. May play a role in cytokinesis
Potential.
Subunit structure: Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation
By similarity. Interacts with SEPT6 and SEPT11. Forms homodimers. Ref.2 Ref.8
Subcellular location: Cytoplasm. Cytoplasm › cytoskeleton. Cytoplasm › cytoskeleton › spindle. Cell projection › cilium › flagellum. Note: At interphase, forms a filamentous structure in the cytoplasm. During anaphase, translocates to the central spindle region and to the midbody during cytokinesis. Found in the sperm annulus. Ref.2 Ref.9
Tissue specificity: Widely expressed. Ref.7
Involvement in disease: Spermatogenic failure 10 (SPGF10) [MIM:614822]: An infertility disorder caused by spermatogenesis defects. It results in decreased sperm motility, concentration, and multiple sperm structural defects. The most prominent feature is a defective sperm annulus, a ring structure that demarcates the midpiece and the principal piece of the sperm tail.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9
Sequence similarities: Belongs to the septin family.
Sequence caution: The sequence AAH24017.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on SEPT12
Similar Products
Product Notes
The SEPT12 sept12 (Catalog #AAA649418) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SEPT12 (Septin 12, FLJ25410, Septin-12) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SEPT12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Dilution: Immunofluorescence: 10ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml. Researchers should empirically determine the suitability of the SEPT12 sept12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MDPLRRSPSP CLSSQPSSPS TPPCEMLGPV GIEAVLDQLK IKAMKMGFEF NIMVVGQSGL GKSTMVNTLF KSKVWKSNPP GLGVPTPQTL QLHSLTHVIE EKGVKLKLTV TDTPGFGDQI NNDNCWDPIL GYINEQYEQY LQEEILITRQ RHIPDTRVHC CVYFVPPTGH CLRPLDIEFL QRLCRTVNVV PVIARADSLT MEEREAFRRR IQQNLRTHCI DVYPQMCFDE DINDKILNSK LRDRIPFAVV GADQEHLVNG RCVLGRKTKW GIIEVENMAH CEFPLLRDLL IRSHLQDLKD ITHNIHYENY RVIRLNESHL LPRGPGWVNL APASPGQLTT PRTFKVCRGA HDDSDDEF. It is sometimes possible for the material contained within the vial of "SEPT12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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