Goat anti-Human Semaphorin 3E Polyclonal Antibody | anti-SEMA3E antibody

Semaphorin 3E (Semaphorin-3E, SEMA3E, Collapsin-5, Coll5, Coll-5, KIAA0331, MSEMAH, Msema-H, M-Sema-H, M-SEMAH, M-SemaK, Sema Domain Immunoglobulin Domain (Ig), Sema Domain Immunoglobulin Domain (Ig) Short Basic Domain Secreted 3E, SEMAH)

Cat. Num. AAA615584

• Gene Names: SEMA3E; SEMAH; coll-5; M-SEMAH; M-SemaK; KIAA0331
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Semaphorin 3E; Polyclonal Antibody; Semaphorin 3E (Semaphorin-3E; SEMA3E; Collapsin-5; Coll5; Coll-5; KIAA0331; MSEMAH; Msema-H; M-Sema-H; M-SEMAH; M-SemaK; Sema Domain Immunoglobulin Domain (Ig); Sema Domain Immunoglobulin Domain (Ig) Short Basic Domain Secreted 3E; SEMAH); Anti -Semaphorin 3E (Semaphorin-3E; anti-SEMA3E antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Semaphorin 3E.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.

• Applicable Applications for anti-SEMA3E antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry.; Dilution: Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Semaphorin 3E. The detection limit for rhSemaphorin 3E is approximately 5ng/lane under non-reducing and reducing conditions.; Immunocytochemistry: 10ug/ml to detect Semaphorin 3E on MDA-MB-453 human breast cancer cells.; Immunohistochemistry: 10ug/ml to detect Semaphorin 3E in cryosections of E13.5 mouse embryos.; Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Semaphorin 3E. The detection limit for rhSemaphorin 3E is approximately 1.0ng/well. In this format, this antibody shows approximately 70% cross-reactivity with rmSemaphorin 3E and less than 5% cross-reactivity with rmSemaphorin 3C and rmSemaphorin 3F.
• Immunogen: NS0-derived rhSemaphorin 3E
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemistry-Semaphorin 3E in Mouse Brain.)

Related Product Information for anti-SEMA3E antibody

Semaphorin 3E (previously SemaH) is a secreted protein belonging to the semaphorin family. Semaphorin 3E is highly expressed by a subset of motor neurons in developing somites, where it acts as a repulsive cue for PlexinD1-expressing endothelial cells of adjacent intersomitic vessels. Like other Semaphorin 3 proteins, the 775 aa human Semaphorin 3E contains a signal sequence, a Semaphorin domain, a C2-like Ig domain and a C-terminal basic domain. Disulfide-linked dimerization and furin-type proteolytic processing increase Semaphorin 3E bioactivity. Mature human and mouse Semaphorin 3E share 90% aa identity.

Product Categories/Family for anti-SEMA3E antibody

Antibodies; Abs to Plexin; Semaphorin

NCBI and Uniprot Product Information

• NCBI GI #: 6912650
• NCBI GeneID: 9723
• NCBI Accession #: NP_036563.1
• NCBI GenBank Nucleotide #: NM_012431.2
• UniProt Accession #: O15041; Q75M94; Q75M97
• Molecular Weight: 89,228 Da
• NCBI Official Full Name: semaphorin-3E isoform 1
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
• NCBI Official Symbol: SEMA3E
• NCBI Official Synonym Symbols: SEMAH; coll-5; M-SEMAH; M-SemaK; KIAA0331
• NCBI Protein Information: semaphorin-3E; OTTHUMP00000206419; OTTHUMP00000206420
• UniProt Protein Name: Semaphorin-3E
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA3E
• UniProt Synonym Gene Names: KIAA0331; SEMAH
• UniProt Entry Name: SEM3E_HUMAN

NCBI Description

Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.

Uniprot Description

SEMA3E: a secreted protein of the semaphorin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q21.11

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: protein binding; receptor activity; semaphorin receptor binding

Biological Process: patterning of blood vessels; axon guidance; regulation of cell shape; negative regulation of angiogenesis; synapse organization and biogenesis; sprouting angiogenesis; negative regulation of cell-matrix adhesion

Disease: Charge Syndrome