Mouse anti-Human PDK4 Monoclonal Antibody | anti-PDK4 antibody

PDK4 (PDK4, PDHK4, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial, Pyruvate dehydrogenase kinase isoform 4) (AP)

Cat. Num. AAA6120878

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: PDK4; Monoclonal Antibody; PDK4 (PDK4; PDHK4; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4; mitochondrial; Pyruvate dehydrogenase kinase isoform 4) (AP); Pyruvate dehydrogenase kinase isoform 4; anti-PDK4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 345CT19.6.2
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PDK4 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1,000; WB: 1:100; Applications are based on unconjugated antibody.
• Immunogen: Purified His-tagged PDK4 protein (fragment).
• Conjugate: AP
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C with the addition of glycerol. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive.

Western Blot (WB)

(PDK4 Antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PDK4 antibody detected the PDK4 protein (arrow).)

Related Product Information for anti-PDK4 antibody

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.

Product Categories/Family for anti-PDK4 antibody

Antibodies; Protein Kinases

References

Lu, Y., et al. J. Lipid Res. 49(12):2582-2589(2008) Wynn, R.M., et al. J. Biol. Chem. 283(37):25305-25315(2008) Cadoudal, T., et al. Diabetes 57(9):2272-2279(2008) Tsintzas, K., et al. J. Clin. Endocrinol. Metab. 92(10):3967-3972(2007) Degenhardt, T., et al. J. Mol. Biol. 372(2):341-355(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 4505693
• NCBI GeneID: 5166
• NCBI Accession #: NP_002603.1
• NCBI GenBank Nucleotide #: NM_002612.3
• UniProt Accession #: Q16654
• Molecular Weight: 46,469 Da
• NCBI Official Full Name: pyruvate dehydrogenase kinase, isozyme 4
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase, isozyme 4
• NCBI Official Symbol: PDK4
• NCBI Protein Information: pyruvate dehydrogenase kinase, isozyme 4; pyruvate dehydrogenase kinase, isoenzyme 4; pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial
• UniProt Protein Name: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial
• UniProt Gene Name: PDK4
• UniProt Synonym Gene Names: PDHK4
• UniProt Entry Name: PDK4_HUMAN

NCBI Description

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq, Jul 2008]

Uniprot Description

PDHK4: an ubiquitously expressed, atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK4 is abundant in heart, skeletal muscle, kidney, and pancreatic islets. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: Kinase, protein; Protein kinase, atypical; EC 2.7.11.2; Mitochondrial; ATYPICAL group; PDHK family

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding; protein kinase activity

Biological Process: regulation of bone resorption; glucose metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; cellular response to starvation; glucose homeostasis; protein amino acid phosphorylation; response to starvation; cellular metabolic process; regulation of fatty acid biosynthetic process; regulation of fatty acid oxidation; insulin receptor signaling pathway; regulation of pH; pyruvate metabolic process