Rabbit anti-Mouse SEC31A Polyclonal Antibody | anti-SEC31A antibody

SEC31A, ID (SEC31A, KIAA0905, SEC31L1, Protein transport protein Sec31A, ABP125, ABP130, SEC31-like protein 1, SEC31-related protein A, Web1-like protein) (PE)

Cat. Num. AAA6345821

• Gene Names: SEC31A; ABP125; ABP130; HSPC275; HSPC334; NEDSOSB; SEC31L1
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SEC31A; Polyclonal Antibody; ID (SEC31A; KIAA0905; SEC31L1; Protein transport protein Sec31A; ABP125; ABP130; SEC31-like protein 1; SEC31-related protein A; Web1-like protein) (PE); Web1-like protein; anti-SEC31A antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SEC31A antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SEC31A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473-502 amino acids from the Central region of human SEC31A.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SEC31A in mouse lung tissue lysates (35ug/lane) using antibodyMBS6004566.)

Related Product Information for anti-SEC31A antibody

The protein encoded by this gene is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII, in the subcellular structures corresponding to the vesicle transport function. An immunodepletion experiment confirmed that this protein is required for ER-Golgi transport. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

Product Categories/Family for anti-SEC31A antibody

Antibodies; Transport Proteins

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Townley, A.K., et al. J. Cell. Sci. 121 (PT 18), 3025-3034 (2008): Stagg, S.M., et al. Cell 134(3):474-484(2008). Shibata, H., et al. J. Biol. Chem. 283(15):9623-9632(2008). Bi, X., et al. Dev. Cell 13(5):635-645(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 41349439
• NCBI GeneID: 22872
• NCBI Accession #: NP_055748.2
• NCBI GenBank Nucleotide #: NM_014933.3
• UniProt Accession #: O94979; Q17RR5; Q5H9P6; Q5XG74; Q659G7; Q6ZU90; Q7LCX9; Q86TJ0; B4DIW6; B7ZKZ7; B7ZL00; H7C2W3
• Molecular Weight: 127,556 Da
• NCBI Official Synonym Full Names: SEC31 homolog A, COPII coat complex component
• NCBI Official Symbol: SEC31A
• NCBI Official Synonym Symbols: ABP125; ABP130; HSPC275; HSPC334; NEDSOSB; SEC31L1
• NCBI Protein Information: protein transport protein Sec31A
• UniProt Protein Name: Protein transport protein Sec31A
• Protein Family: Protein transport protein
• UniProt Gene Name: SEC31A
• UniProt Synonym Gene Names: KIAA0905; SEC31L1
• UniProt Entry Name: SC31A_HUMAN

NCBI Description

The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]