Rabbit anti-Human SEC13 Polyclonal Antibody | anti-SEC13 antibody

SEC13, ID (SEC13, D3S1231E, SEC13L1, SEC13R, Protein SEC13 homolog, SEC13-like protein 1, SEC13-related protein) (AP)

Cat. Num. AAA6345712

• Gene Names: SEC13; SEC13R; npp-20; SEC13L1; D3S1231E
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SEC13; Polyclonal Antibody; ID (SEC13; D3S1231E; SEC13L1; SEC13R; Protein SEC13 homolog; SEC13-like protein 1; SEC13-related protein) (AP); SEC13-related protein; anti-SEC13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 322

• Applicable Applications for anti-SEC13 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SEC13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-104 amino acids from the Central region of human SEC13.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SEC13 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SEC13 antibody detected the SEC13 protein (arrow).)

Flow Cytometry (FC/FACS)

(SEC13 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SEC13 antibody

The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found.

Product Categories/Family for anti-SEC13 antibody

Antibodies; Transport Proteins

References

Nielsen, A.L. Biochem. Biophys. Res. Commun. 388(3):571-575(2009). Townley, A.K., et al. J. Cell. Sci. 121 (PT 18), 3025-3034 (2008): Stagg, S.M., et al. Cell 134(3):474-484(2008). Hsia, K.C., et al. Cell 131(7):1313-1326(2007). Glavy, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 104(10):3811-3816(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 34335134
• NCBI GeneID: 6396
• NCBI Accession #: NP_899195.1
• NCBI GenBank Nucleotide #: NP_899195.1
• UniProt Accession #: P55735
• NCBI Official Full Name: protein SEC13 homolog isoform 1
• NCBI Official Synonym Full Names: SEC13 homolog, nuclear pore and COPII coat complex component
• NCBI Official Symbol: SEC13
• NCBI Official Synonym Symbols: SEC13R; npp-20; SEC13L1; D3S1231E
• NCBI Protein Information: protein SEC13 homolog
• UniProt Protein Name: Protein SEC13 homolog
• Protein Family: Protein transport protein
• UniProt Gene Name: SEC13
• UniProt Synonym Gene Names: D3S1231E; SEC13L1; SEC13R
• UniProt Entry Name: SEC13_HUMAN

NCBI Description

The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2008]

Uniprot Description

SEC13: Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. Belongs to the WD repeat SEC13 family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p25-p24

Cellular Component: kinetochore; Golgi membrane; endoplasmic reticulum membrane; nuclear envelope; cytosol

Molecular Function: protein binding

Biological Process: COPII coating of Golgi vesicle; intracellular protein transport; antigen processing and presentation of peptide antigen via MHC class I; ER to Golgi vesicle-mediated transport; mRNA transport; cellular protein metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; protein amino acid N-linked glycosylation via asparagine; mitotic cell cycle; post-translational protein modification