Fibrillin 3 Recombinant Protein | FBN3 recombinant protein

Recombinant Fibrillin 3 (FBN3)

Cat. Num. AAA2030174

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Fibrillin 3; Recombinant Fibrillin 3 (FBN3); FBN3 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 5.6 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ATLNQTID ICRHFTNLCL NGRCLPTPSS YRCECNVGYT QDVRGECIDV DECTSSPCHH GDCVNIPGTY HCRCYPGFQA TPTRQACVDV DECIVSGGLC HLGRCVNTEG SFQCVCNAGF ELSPDGKNCV DHNECATSTM CVNGVCLNED GSFSCLCKPG FLLAPGGHYC MDIDECQTPG ICVNGHCTNT EGSFRCQCLG GLAVGTDGRV CVDTHVRSTC YGAIEKGSCARPFPGTVTKS ECCCANPDHG FGEPCQLCPA KDSAEFQ
• Sequence Length: 2809

• Applicable Applications for FBN3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 32.1kDa
• Accurate Molecular Mass (KD): 34kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Gln1~Pro124
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 46559348
• NCBI GeneID: 84467
• Molecular Weight: 300,356 Da
• NCBI Official Full Name: fibrillin 3
• NCBI Official Synonym Full Names: fibrillin 3
• NCBI Official Symbol: FBN3
• NCBI Protein Information: fibrillin-3
• UniProt Protein Name: Fibrillin-3
• Protein Family: Fibrillin
• UniProt Gene Name: FBN3
• UniProt Synonym Gene Names: KIAA1776
• UniProt Entry Name: FBN3_HUMAN

NCBI Description

This gene encodes a protein that belongs to the fibrillin gene family. Fibrillins are extracellular matrix molecules that assemble into microfibrils in many connective tissues. This gene is most highly expressed in fetal tissues and its protein product is localized to extracellular microfibrils of developing skeletal elements, skin, lung, kidney, and skeletal muscle. This gene is potentially involved in Weill-Marchesani syndrome. While several transcript variants may exist for this gene, their full-length natures have not been described to date. [provided by RefSeq, Jul 2008]

Uniprot Description

FBN3: Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin- containing microfibrils provide long-term force bearing structural support. Belongs to the fibrillin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: proteinaceous extracellular matrix

Molecular Function: extracellular matrix structural constituent; calcium ion binding