Rabbit SDHA Polyclonal Antibody | anti-SDHA antibody

SDHA (R600) Polyclonal Antibody

Cat. Num. AAA3003115

• Gene Names: SDHA; FP; PGL5; SDH1; SDH2; SDHF; CMD1GG
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SDHA; Polyclonal Antibody; SDHA (R600) Polyclonal Antibody; Succinate dehydrogenase [ubiquinone] flavoprotein subunit; mitochondrial; Flavoprotein subunit of complex II; Fp; SDH2; SDHF; anti-SDHA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SDHA (R600) polyclonal antibody detects endogenous levels of SDHA protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 664

• Applicable Applications for anti-SDHA antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 560-610 of Human SDHA.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SDHA (R600) pAb at 1:2000 dilutionLane1:The Heart tissue lysate of Rat (20ug) Lane2:The Testis tissue lysate of Mouse (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug))

Related Product Information for anti-SDHA antibody

In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB), and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations are associated with juvenile encephalopathy as well as Leigh Syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma.

NCBI and Uniprot Product Information

• NCBI GI #: 1169337
• NCBI GeneID: 6389
• UniProt Accession #: P31040
• Molecular Weight: ~ 72kDa
• NCBI Official Full Name: Succinate dehydrogenase
• NCBI Official Synonym Full Names: succinate dehydrogenase complex flavoprotein subunit A
• NCBI Official Symbol: SDHA
• NCBI Official Synonym Symbols: FP; PGL5; SDH1; SDH2; SDHF; CMD1GG
• NCBI Protein Information: succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
• UniProt Protein Name: Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
• Protein Family: Succinate dehydrogenase
• UniProt Gene Name: SDHA
• UniProt Synonym Gene Names: SDH2; SDHF; Fp
• UniProt Entry Name: SDHA_HUMAN

NCBI Description

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

SDHA: is the catalytic subunit of succinate dehydrogenase (SDH) complex II of the mitochondrial electron transport chain. Complex II contains four subunits: the flavoprotein catalytic subunit SDHA, iron-sulfur protein SDHB, and a cytochrome b560 composed of SDHC and SDHD. Interaction with SDH5 is required for FAD attachment. Responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHA cause defective mitochondrial oxidative phosphorylation, giving rise to heterogeneous clinical symptoms ranging from isolated organ dysfunction to multisystem disorder. Acetylation of SDHA may control entry of the substrate into the active site, thus regulating its enzymatic activity. Acetylated SDHA may be a SIRT3 substrate SIRT3 is a mitochondrial NAD(+)-dependent deacetylase. Increased succinate levels as a consequence of SDH deficiency inhibit hypoxia inducible factor-1alpha (HIF-1alpha) prolyl hydroxylases leading to sustained HIF-1alpha expression in tumours. Defects in SDHA cause of Leigh syndrome, a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.

Protein type: Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.3.5.1; Oxidoreductase; Tumor suppressor; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 5p15

Cellular Component: mitochondrial respiratory chain complex II; mitochondrion; mitochondrial inner membrane

Molecular Function: protein binding; succinate dehydrogenase (ubiquinone) activity; FAD binding; succinate dehydrogenase activity

Biological Process: succinate metabolic process; cellular metabolic process; nervous system development; tricarboxylic acid cycle

Disease: Mitochondrial Complex Ii Deficiency; Leigh Syndrome; Paragangliomas 5; Cardiomyopathy, Dilated, 1gg