Western Blot (WB)
(Anti-SDHA antibody, MBS175744, Western blottingLane 1: Rat Heart Tissue LysateLane 2: COLO320 Cell Lysate )
Rabbit SDHA Polyclonal Antibody | anti-SDHA antibody
Anti-SDHA antibody
Western Blot (WB)
(Anti-SDHA antibody, MBS175744, Western blottingLane 1: Rat Heart Tissue LysateLane 2: COLO320 Cell Lysate )
Western Blot (WB)
(Anti-SDHA antibody, MBS175744, Western blottingLane 1: Rat Heart Tissue LysateLane 2: COLO320 Cell Lysate )
Background: SDHA(Succinate dehydrogenase complex, subunit a, flavoprotein), also called SDH1, HOMOLOG OF, is a protein that in humans is encoded by the SDHA gene. The SDHA gene is mapped on 5p15.33. The SDHA gene is highly polymorphic. The gene that codes for the SDHA protein is nuclear, even though the protein is located in the inner membrane of the mitochondria. Heterozygous carriers of an SDHA mutation do not developparagangliomas as has been seen for mutations in the other subunits. In vitro functional expression studies in the yeast homolog showed that the mutation resulted in a loss of SDH activity and rendered the mutant SDHA protein more susceptible to proteolysis. The findings indicated that SDHA, like other SDH subunits, can act as a tumor suppressor gene.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
Uniprot Description
SDHA: is the catalytic subunit of succinate dehydrogenase (SDH) complex II of the mitochondrial electron transport chain. Complex II contains four subunits: the flavoprotein catalytic subunit SDHA, iron-sulfur protein SDHB, and a cytochrome b560 composed of SDHC and SDHD. Interaction with SDH5 is required for FAD attachment. Responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHA cause defective mitochondrial oxidative phosphorylation, giving rise to heterogeneous clinical symptoms ranging from isolated organ dysfunction to multisystem disorder. Acetylation of SDHA may control entry of the substrate into the active site, thus regulating its enzymatic activity. Acetylated SDHA may be a SIRT3 substrate SIRT3 is a mitochondrial NAD(+)-dependent deacetylase. Increased succinate levels as a consequence of SDH deficiency inhibit hypoxia inducible factor-1alpha (HIF-1alpha) prolyl hydroxylases leading to sustained HIF-1alpha expression in tumours. Defects in SDHA cause of Leigh syndrome, a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
Protein type: Energy Metabolism - oxidative phosphorylation; Mitochondrial; EC 1.3.5.1; Carbohydrate Metabolism - citrate (TCA) cycle; Oxidoreductase; Tumor suppressor
Chromosomal Location of Human Ortholog: 5p15
Cellular Component: mitochondrial respiratory chain complex II; mitochondrion; mitochondrial inner membrane
Molecular Function: succinate dehydrogenase (ubiquinone) activity; protein binding; FAD binding; succinate dehydrogenase activity
Biological Process: cellular metabolic process; succinate metabolic process; nervous system development; tricarboxylic acid cycle
Disease: Mitochondrial Complex Ii Deficiency; Leigh Syndrome; Paragangliomas 5; Cardiomyopathy, Dilated, 1gg
Research Articles on SDHA
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Product Notes
The SDHA sdha (Catalog #AAA175744) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SDHA antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SDHA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the SDHA sdha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SDHA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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