Rabbit anti-Human SCGN Polyclonal Antibody | anti-SCGN antibody

SCGN, NT (Secretagogin, SECRET) (Biotin)

Cat. Num. AAA6498672

• Gene Names: SCGN; SEGN; CALBL; SECRET; setagin; DJ501N12.8
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SCGN; Polyclonal Antibody; NT (Secretagogin; SECRET) (Biotin); anti-SCGN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SCGN.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SCGN antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human SCGN (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of *SCGN (arrow) using rabbit polyclonal *SCGN Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the *SCGN gene.)

Immunohistochemistry (IHC)

(SCGN Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCGN Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SCGN) Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SCGN antibody

Antibodies; Disease Markers

References

1. Skovhus, K.V., Scand. J. Immunol. 64(6), 639-645 (2006). 2. Zierhut, B., Biochem. Biophys. Res. Commun. 329(4), 1193-1199 (2005). 3. Mungall, A.J., Nature 425(6960), 805-811 (2003). 4. Zhan, X., Pituitary 6 (4), 189-202 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 15055537
• NCBI GeneID: 10590
• NCBI Accession #: NP_008929
• NCBI GenBank Nucleotide #: NM_006998.3
• UniProt Accession #: O76038; Q5VV44; Q96QV7; Q9UJF6; A8K0B2
• Molecular Weight: 34.2 kDa (296aa), confirmed by MALDI-TOF
• NCBI Official Full Name: secretagogin
• NCBI Official Synonym Full Names: secretagogin, EF-hand calcium binding protein
• NCBI Official Symbol: SCGN
• NCBI Official Synonym Symbols: SEGN; CALBL; SECRET; setagin; DJ501N12.8
• NCBI Protein Information: secretagogin
• UniProt Protein Name: Secretagogin
• Protein Family: Secretagogin
• UniProt Gene Name: SCGN
• UniProt Synonym Gene Names: SECRET
• UniProt Entry Name: SEGN_HUMAN

NCBI Description

The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

SCGN:

Chromosomal Location of Human Ortholog: 6p22.3-p22.1

Cellular Component: cytoplasm; extracellular region

Molecular Function: calcium ion binding