Mouse anti-Human IRS4 Monoclonal Antibody | anti-IRS4 antibody

IRS4 (Insulin Receptor Substrate 4, IRS-4, 160kD Phosphotyrosine Protein, py160, Phosphoprotein of 160kD, pp160) (Biotin)

Cat. Num. AAA6142509

• Gene Names: IRS4; CHNG9; IRS-4; PY160
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IRS4; Monoclonal Antibody; IRS4 (Insulin Receptor Substrate 4; IRS-4; 160kD Phosphotyrosine Protein; py160; Phosphoprotein of 160kD; pp160) (Biotin); anti-IRS4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 5B9
• Specificity: Recognizes human IRS4.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 1257

• Applicable Applications for anti-IRS4 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa850-951 from IRS4 (NP_003595) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: DPKDAASKPSGEGSFSKPGDGGSPSKPSDHEPPKNKAKRPNRLSFITKGYKIKPKPQKPTHEQREADSSSDYVNMDFTKRESNTPAPSTQGLPDSWGIIAE*
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (37.22kD).)

Western Blot (WB)

(IRS4 monoclonal antibody Western Blot analysis of IRS4 expression in HeLa.)

Related Product Information for anti-IRS4 antibody

IRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation.

Product Categories/Family for anti-IRS4 antibody

Antibodies; Protein Kinase Substrates

NCBI and Uniprot Product Information

• NCBI GI #: 4504733
• NCBI GeneID: 8471
• NCBI Accession #: NP_003595
• NCBI GenBank Nucleotide #: NM_003604
• UniProt Accession #: O14654
• NCBI Official Full Name: insulin receptor substrate 4
• NCBI Official Synonym Full Names: insulin receptor substrate 4
• NCBI Official Symbol: IRS4
• NCBI Official Synonym Symbols: CHNG9; IRS-4; PY160
• NCBI Protein Information: insulin receptor substrate 4
• UniProt Protein Name: Insulin receptor substrate 4
• Protein Family: Insulin receptor substrate
• UniProt Gene Name: IRS4
• UniProt Synonym Gene Names: IRS-4; py160; pp160

NCBI Description

IRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation.. [provided by RefSeq, Jul 2008]

Uniprot Description

Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGF1 signaling pathway by suppressing the function of IRS1 and IRS2.