Immunohistochemistry (IHC)
(Immunochemical staining of human SCGN in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Human SCGN Polyclonal Antibody | anti-SCGN antibody
Anti-SCGN Antibody, Rabbit Polyclonal
Gene Names
SCGN; SEGN; CALBL; SECRET; setagin; DJ501N12.8
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
SCGN; Polyclonal Antibody; Anti-SCGN Antibody; Rabbit Polyclonal; SCGN/Secretagogin Antibody; Rabbit PAb; Antigen Affinity Purified; secretagogin; EF-hand calcium binding protein; Anti-CALBL Antibody; Anti-DJ501N12.8 Antibody; Anti-SECRET Antibody; Anti-SEGN Antibody; Anti-setagin Antibody; anti-SCGN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SCGN/Secretagogin
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SCGN antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IHC-P: 1:500-1:2000
Immunogen
Recombinant Human SCGN/Secretagogin protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human SCGN/Secretagogin (rh SCGN/Secretagogin). SCGN/Secretagogin specific IgG was purified by Human SCGN/Secretagogin affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human SCGN in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human SCGN in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-SCGN antibody
Secretagogin, also known as SCGN, is a secreted protein that is detectable in human serum after ischemic neuronal damage. It is a recently described calcium-binding protein. Secretagogin/SCGN is expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression of Secretagogin/SCGN is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons. Secretagogin/SCGN is widely expressed in prostatic adenocarcinoma as opposed to adenocarcinomas in other organs. The function of Secretagogin/SCGN is unknown, but it has been suggested in beta-cells to influence calcium-influx and has been observed downregulated in diabetes-prone BB rat islets exposed to cytokines. Secretagogin/SCGN is involved in the calcium metabolism of tumour cells and endothelial cells in a subset of neoplasms of the brain and its coverings. Secretagogin/SCGN is also a novel marker for neuroendocrine differentiation.
References
Birkenkamp-D, K. et al., 2005, Neuroendocrinology. 82 (2):121-38. Skovhus, K.V. et al., 2006, Scand J Immunol. 64 (6):639-45. Pipp, I. et al., 2007, APMIS.115 (4):319-26. Rogstam, A. et al., 2007, Biochem J. 401 (1):353-63. Adolf, K. et al., 2007, Prostate. 67 (5):472-84.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,040 Da
NCBI Official Full Name
secretagogin
NCBI Official Synonym Full Names
secretagogin, EF-hand calcium binding protein
NCBI Official Symbol
SCGN
NCBI Official Synonym Symbols
SEGN; CALBL; SECRET; setagin; DJ501N12.8
NCBI Protein Information
secretagogin
UniProt Protein Name
Secretagogin
Protein Family
UniProt Gene Name
SCGN
UniProt Synonym Gene Names
SECRET
UniProt Entry Name
SEGN_HUMAN
NCBI Description
The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq, Jul 2008]
Uniprot Description
SCGN:
Chromosomal Location of Human Ortholog: 6p22.3-p22.1
Cellular Component: cytoplasm; extracellular region
Molecular Function: calcium ion binding
Research Articles on SCGN
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Product Notes
The SCGN scgn (Catalog #AAA8106422) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SCGN Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SCGN can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000. Researchers should empirically determine the suitability of the SCGN scgn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SCGN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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