SCGN recombinant protein

SCGN Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121162

• Gene Names: SCGN; SEGN; CALBL; SECRET; setagin; DJ501N12.8
• Purity: >97% as determined by SDS-PAGE
• Synonyms: SCGN; SCGN Protein; Human; Recombinant (His Tag); Human SCGN/Secretagogin Protein (His Tag); CALBL Protein; DJ501N12.8 Protein; SECRET Protein; SEGN Protein; setagin Protein; secretagogin; EF-hand calcium binding protein; SCGN recombinant protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Asp2-Pro276

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human SCGN (NP_008929.2)(Asp 2-Pro 276) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SCGN recombinant protein

Background: Secretagogin, also known as SCGN, is a secreted protein that is detectable in human serum after ischemic neuronal damage. It is a recently described calcium-binding protein. Secretagogin/SCGN is expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression of Secretagogin/SCGN is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons. Secretagogin/SCGN is widely expressed in prostatic adenocarcinoma as opposed to adenocarcinomas in other organs. The function of Secretagogin/SCGN is unknown, but it has been suggested in beta-cells to influence calcium-influx and has been observed downregulated in diabetes-prone BB rat islets exposed to cytokines. Secretagogin/SCGN is involved in the calcium metabolism of tumour cells and endothelial cells in a subset of neoplasms of the brain and its coverings. Secretagogin/SCGN is also a novel marker for neuroendocrine differentiation.

References

Birkenkamp-D,K. et al., 2005, Neuroendocrinology. 82 (2):121-38. Skovhus,K.V. et al., 2006, Scand J Immunol. 64 (6):639-45. Pipp,I. et al., 2007, APMIS.115 (4):319-26. Rogstam, A. et al., 2007, Biochem J. 401 (1):353-63. Adolf,K. et al., 2007, Prostate. 67 (5):472-84.

NCBI and Uniprot Product Information

• NCBI GI #: 15055537
• NCBI GeneID: 10590
• NCBI Accession #: NP_008929.2
• NCBI GenBank Nucleotide #: NM_006998.3
• UniProt Accession #: O76038; Q5VV44; Q96QV7; Q9UJF6; A8K0B2
• Molecular Weight: 32,040 Da
• NCBI Official Full Name: secretagogin
• NCBI Official Synonym Full Names: secretagogin, EF-hand calcium binding protein
• NCBI Official Symbol: SCGN
• NCBI Official Synonym Symbols: SEGN; CALBL; SECRET; setagin; DJ501N12.8
• NCBI Protein Information: secretagogin
• UniProt Protein Name: Secretagogin
• Protein Family: Secretagogin
• UniProt Gene Name: SCGN
• UniProt Synonym Gene Names: SECRET
• UniProt Entry Name: SEGN_HUMAN

NCBI Description

The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

SCGN:

Chromosomal Location of Human Ortholog: 6p22.3-p22.1

Cellular Component: cytoplasm; extracellular region

Molecular Function: calcium ion binding