anti-Human, Mouse NFAT5 Antibody | anti-NFAT5 antibody

NFAT5 (Phospho-Ser1197) Antibody

Cat. Num. AAA8509818

• Gene Names: NFAT5; NFATZ; OREBP; NF-AT5; NFATL1; TONEBP
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, ELISA
• Purity: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: NFAT5; NFAT5 (Phospho-Ser1197) Antibody; KIAA0827; NF-AT5; T cell transcription factor NFAT5; TonE-binding protein; nuclear factor of activated T cells 5; tonicity-responsive enhancer-binding protein; anti-NFAT5 antibody

• Reactivity: Human, Mouse
• Specificity: NFAT5 (Phospho-Ser1197) antibody detects endogenous levels of NFAT5 only when phosphorylated at serine 1197.
• Purity/Purification: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1548

• Applicable Applications for anti-NFAT5 antibody: Immunofluorescence (IF), ELISA (EIA)
• Application Notes: IF: 1:100-1:500; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human NFAT5 around the phosphorylation site of serine 1197 (M-L-SP-Q-E).
• All Sites: Human: Ser1197; Mouse: Ser1197
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

(P-peptide-+)

Immunofluorescence (IF)

(Immunofluorescence analysis of COS7 cells, using NFAT5 (Phospho-Ser1197) antibody. NFAT5 (Phospho-Ser1197) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

References

Jonathan H. Esensten, Nucleic Acids Res., Jul 2005; 33: 3845-3854. Jason Trama, J. Immunol., Nov 2002; 169: 5477. Cristina López-Rodríguez, PNAS, Feb 2004; 101: 2392.

NCBI and Uniprot Product Information

• NCBI GI #: 164419746
• NCBI GeneID: 10725
• NCBI Accession #: NP_001106649.1
• NCBI GenBank Nucleotide #: NM_001113178.2
• UniProt Accession #: O94916; O95693; Q7LA65; Q969Q8; Q96QH3; Q9UN18; A2RRB4; A6H8V5; E9PHR7
• Molecular Weight: 167,808 Da
• NCBI Official Full Name: nuclear factor of activated T-cells 5 isoform d
• NCBI Official Synonym Full Names: nuclear factor of activated T cells 5
• NCBI Official Symbol: NFAT5
• NCBI Official Synonym Symbols: NFATZ; OREBP; NF-AT5; NFATL1; TONEBP
• NCBI Protein Information: nuclear factor of activated T-cells 5
• UniProt Protein Name: Nuclear factor of activated T-cells 5
• Protein Family: Nuclear factor of activated T-cells
• UniProt Gene Name: NFAT5
• UniProt Synonym Gene Names: KIAA0827; TONEBP; TonEBP

NCBI Description

The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Mediates the transcriptional response to hypertonicity (PubMed:10051678). Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed:22266867). Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed:10377394).