Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of Neuro-2a cells using anti-SCG10/STMN2 antibody (MBS1753671).Overlay histogram showing Neuro-2a cells stained with MBS1753671 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCG10/STMN2 Antibody (MBS1753671, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Rabbit SCG10/STMN2 Polyclonal Antibody | anti-STMN2 antibody
Anti-SCG10/STMN2 Antibody
IHC-P: 2-5ug/ml|Mouse, Rat|
ICC/IF: 5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human, Mouse|
Direct ELISA: 0.1-0.5ug/ml|Human|
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of Neuro-2a cells using anti-SCG10/STMN2 antibody (MBS1753671).Overlay histogram showing Neuro-2a cells stained with MBS1753671 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCG10/STMN2 Antibody (MBS1753671, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of Neuro-2a cells using anti-SCG10/STMN2 antibody (MBS1753671).Overlay histogram showing Neuro-2a cells stained with MBS1753671 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCG10/STMN2 Antibody (MBS1753671, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of U20S cells using anti-SCG10/STMN2 antibody (MBS1753671).Overlay histogram showing U20S cells stained with MBS1753671 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCG10/STMN2 Antibody (MBS1753671, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of U20S cells using anti-SCG10/STMN2 antibody (MBS1753671).Overlay histogram showing U20S cells stained with MBS1753671 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCG10/STMN2 Antibody (MBS1753671, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Western Blot (WB)
(Figure 1. Western blot analysis of SCG10/STMN2 using anti-SCG10/STMN2 antibody (MBS1753671).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysatesLane 2: rat brain tissue lysatesLane 3: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SCG10/STMN2 antigen affinity purified polyclonal antibody (Catalog # MBS1753671) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SCG10/STMN2 at approximately 21KD. The expected band size for SCG10/STMN2 is at 21KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of SCG10/STMN2 using anti-SCG10/STMN2 antibody (MBS1753671).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysatesLane 2: rat brain tissue lysatesLane 3: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SCG10/STMN2 antigen affinity purified polyclonal antibody (Catalog # MBS1753671) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SCG10/STMN2 at approximately 21KD. The expected band size for SCG10/STMN2 is at 21KD. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of SCG10/STMN2 using anti-SCG10/STMN2 antibody (MBS1753671).SCG10/STMN2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SCG10/STMN2 Antibody (MBS1753671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of SCG10/STMN2 using anti-SCG10/STMN2 antibody (MBS1753671).SCG10/STMN2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SCG10/STMN2 Antibody (MBS1753671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 5. IHC analysis of SCG10/STMN2 using anti-SCG10/STMN2 antibody (MBS1753671).SCG10/STMN2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SCG10/STMN2 Antibody (MBS1753671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 5. IHC analysis of SCG10/STMN2 using anti-SCG10/STMN2 antibody (MBS1753671).SCG10/STMN2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SCG10/STMN2 Antibody (MBS1753671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunofluorescence (IF)
(Figure 6. IF analysis of SCG10/STMN2 using anti- SCG10/STMN2 antibody (MBS1753671).SCG10/STMN2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- SCG10/STMN2 Antibody (MBS1753671) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Immunofluorescence (IF)
(Figure 6. IF analysis of SCG10/STMN2 using anti- SCG10/STMN2 antibody (MBS1753671).SCG10/STMN2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- SCG10/STMN2 Antibody (MBS1753671) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
2. Okazaki, T., Yoshida, B. N., Avraham, K. B., Wang, H., Wuenschell, C. W., Jenkins, N. A., Copeland, N. G., Anderson, D. J., Mori, N. Molecular diversity of the SCG10/stathmin gene family in the mouse. Genomics 18: 360-373, 1993. Note: Erratum: Genomics 21: 298 only, 1994.
3. Stein, R., Mori, N., Matthews, K., Lo, L. -C., Anderson, D. J. The NGF-inducible SCG10 mRNA encodes a novel membrane-bound protein present in growth cones and abundant in developing neurons. Neuron 1: 463-476, 1988.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the stathmin family of phosphoproteins. Stathmin proteins function in microtubule dynamics and signal transduction. The encoded protein plays a regulatory role in neuronal growth and is also thought to be involved in osteogenesis. Reductions in the expression of this gene have been associated with Down's syndrome and Alzheimer's disease. Alternatively spliced transcript variants have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Nov 2010]
Uniprot Description
STMN2: a cytoskeletal protein that may play a role in neuronal differentiation, and in modulating membrane interaction with the cytoskeleton during neurite outgrowth. Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. Neuron specific.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 8q21.13
Cellular Component: Golgi apparatus; neuron projection; growth cone; cell soma; membrane; perinuclear region of cytoplasm; axon; lamellipodium; cytoplasm; vesicle; endosome
Molecular Function: tubulin binding; protein binding; calcium-dependent protein binding
Biological Process: positive regulation of microtubule depolymerization; negative regulation of microtubule depolymerization; negative regulation of microtubule polymerization
Research Articles on STMN2
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Product Notes
The STMN2 stmn2 (Catalog #AAA1753671) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SCG10/STMN2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SCG10/STMN2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| IHC-P: 2-5ug/ml|Mouse, Rat| ICC/IF: 5ug/ml|Human| FC/FACS/FCM: 1-3ug/1x106 cells|Human, Mouse| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the STMN2 stmn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SCG10/STMN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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