Galectin 1 (GAL1) Recombinant Protein | GAL1 recombinant protein

Recombinant Galectin 1 (GAL1)

Cat. Num. AAA2010756

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Galectin 1 (GAL1); Recombinant Galectin 1 (GAL1); GAL1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-MACGLVASNL NLKPGECLKV RGELAPDAKS FVLNLGKDSN NLCLHFNPRF NAHGDANTIV CNSKDDGTWG TEQRETAFPF QPGSITEVCI TFDQADLTIK LPDGHEFKFP NRLNMEAINY MAADGDFKIK CVAFE
• Sequence Length: 135

• Applicable Applications for GAL1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Met1~Glu135 (Accession # P11762) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 9845261
• NCBI GeneID: 56646
• NCBI Accession #: NP_063969.1
• NCBI GenBank Nucleotide #: NM_019904.1
• UniProt Accession #: P11762
• Molecular Weight: 16.1kDa
• NCBI Official Full Name: galectin-1
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 1
• NCBI Official Symbol: Lgals1
• NCBI Protein Information: galectin-1; gal-1; RL 14.5; galaptin; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; lectin, galactose binding, soluble 1; beta-galactoside-binding lectin L-14-I
• UniProt Protein Name: Galectin-1
• Protein Family: Gallinacin
• UniProt Gene Name: Lgals1
• UniProt Synonym Gene Names: Gal-1
• UniProt Entry Name: LEG1_RAT

NCBI Description

lectin that binds beta-galactoside [RGD, Feb 2006]

Uniprot Description

Galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Protein type: Cell adhesion

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cell surface; cytoplasm; intracellular; nucleus

Molecular Function: signal transducer activity; protein homodimerization activity; lactose binding; laminin binding; galactoside binding; glycoprotein binding; carbohydrate binding

Biological Process: myoblast differentiation; response to drug; plasma cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; T cell costimulation; positive regulation of erythrocyte aggregation; response to axon injury; signal transduction; multicellular organismal response to stress