Bovine Complement 3a (C3a) ELISA Kit | C3a elisa kit

Bovine Complement 3a (C3a) ELISA Kit

Cat. Num. AAA2608636

• Gene Names: C3AR1; AZ3B; C3AR; HNFAG09
• Reactivity: Bovine
• Synonyms: Complement 3a (C3a); Bovine Complement 3a (C3a) ELISA Kit; C3a elisa kit

• Reactivity: Bovine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 482

• Samples: Bovine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for C3a elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Bovine C3a monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4757888
• NCBI GeneID: 719
• NCBI Accession #: NP_004045.1
• NCBI GenBank Nucleotide #: NM_004054.3
• Molecular Weight: 53,864 Da
• NCBI Official Full Name: C3a anaphylatoxin chemotactic receptor
• NCBI Official Synonym Full Names: complement C3a receptor 1
• NCBI Official Symbol: C3AR1
• NCBI Official Synonym Symbols: AZ3B; C3AR; HNFAG09
• NCBI Protein Information: C3a anaphylatoxin chemotactic receptor
• UniProt Protein Name: C3a anaphylatoxin chemotactic receptor
• UniProt Gene Name: C3AR1
• UniProt Synonym Gene Names: AZ3B; C3R1; HNFAG09; C3AR; C3a-R
• UniProt Entry Name: C3AR_HUMAN

NCBI Description

C3a is an anaphylatoxin released during activation of the complement system. The protein encoded by this gene is an orphan G protein-coupled receptor for C3a. Binding of C3a by the encoded receptor activates chemotaxis, granule enzyme release, superoxide anion production, and bacterial opsonization. [provided by RefSeq, May 2016]

Uniprot Description

C3aR: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: blood circulation; elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); inflammatory response; leukocyte migration; regulation of complement activation