Rabbit anti-Human SAP18 Polyclonal Antibody | anti-SAP18 antibody

SAP18, ID (SAP18, Histone deacetylase complex subunit SAP18, 18kD Sin3-associated polypeptide, 2HOR0202, Cell growth-inhibiting gene 38 protein, Sin3-associated polypeptide p18) (FITC)

Cat. Num. AAA6345000

• Gene Names: SAP18; SAP18P; 2HOR0202
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SAP18; Polyclonal Antibody; ID (SAP18; Histone deacetylase complex subunit SAP18; 18kD Sin3-associated polypeptide; 2HOR0202; Cell growth-inhibiting gene 38 protein; Sin3-associated polypeptide p18) (FITC); Sin3-associated polypeptide p18; anti-SAP18 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SAP18 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SAP18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-86 amino acids from the Central region of human SAP18.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SAP18 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SAP18 antibody detected the SAP18 protein (arrow).)

Related Product Information for anti-SAP18 antibody

Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2.

Product Categories/Family for anti-SAP18 antibody

Antibodies; Histones

References

Sorin, M., et al. PLoS Pathog. 5 (6), E1000463 (2009): McCallum, S.A., et al. Biochemistry 45(39):11974-11982(2006). Joselin, A.P., et al. J. Biol. Chem. 281(18):12475-12484(2006). Schwerk, C., et al. Mol. Cell. Biol. 23(8):2981-2990(2003). Koipally, J., et al. EMBO J. 18(11):3090-3100(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 215490089
• NCBI GeneID: 10284
• NCBI Accession #: NP_005861.2
• NCBI GenBank Nucleotide #: NM_005870.4
• UniProt Accession #: O00422; Q2TTR4; Q6IAW9; Q8N606; Q9UF14; B2R494
• Molecular Weight: 17,561 Da
• NCBI Official Full Name: histone deacetylase complex subunit SAP18
• NCBI Official Synonym Full Names: Sin3A-associated protein, 18kDa
• NCBI Official Symbol: SAP18
• NCBI Official Synonym Symbols: SAP18P; 2HOR0202
• NCBI Protein Information: histone deacetylase complex subunit SAP18; 18 kDa Sin3-associated polypeptide; cell growth inhibiting protein 38; cell growth-inhibiting gene 38 protein; cell growth-inhibiting protein 38; histone deacetlyase complex subunit SAP18; sin3-associated polypep
• UniProt Protein Name: Histone deacetylase complex subunit SAP18
• Protein Family: Zinc finger A20 domain-containing stress-associated protein
• UniProt Gene Name: SAP18
• UniProt Entry Name: SAP18_HUMAN

NCBI Description

Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2008]

Uniprot Description

SAP18: Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Belongs to the SAP18 family.

Chromosomal Location of Human Ortholog: 13q12.11

Cellular Component: nucleoplasm; histone deacetylase complex; cytoplasm; nuclear speck

Molecular Function: protein binding; transcription corepressor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; negative regulation of nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; positive regulation of apoptosis; RNA splicing; gene expression; mRNA processing; regulation of gene expression, epigenetic; regulation of alternative nuclear mRNA splicing, via spliceosome