Western Blot (WB)
(All lanes : Anti-Runx1 Antibody (C-term) at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: NIH/3T3 whole cell lysatesLane 3: mouse thymus lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 49 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, mouse Runx1 Polyclonal Antibody | anti-Runx1 antibody
Mouse Runx1 Antibody (C-term)
Western Blot (WB)
(All lanes : Anti-Runx1 Antibody (C-term) at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: NIH/3T3 whole cell lysatesLane 3: mouse thymus lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 49 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Runx1 Antibody (C-term) at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: NIH/3T3 whole cell lysatesLane 3: mouse thymus lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 49 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Bae S.-C.,et al.Mol. Cell. Biol. 14:3242-3252(1994).
Calabi F.,et al.Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
Tsuji K.,et al.Biochem. Biophys. Res. Commun. 274:171-176(2000).
Fujita Y.,et al.Biochem. Biophys. Res. Commun. 281:1248-1255(2001).
NCBI and Uniprot Product Information
Uniprot Description
AML1: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation. Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Isoform AML-1L can neither bind DNA nor heterodimerize. Interacts with TLE1 and ALYREF/THOC4. Interacts with ELF1, ELF2 and SPI1. Interacts via its Runt domain with the ELF4 N-terminal region. Interaction with ELF2 isoform 2 (NERF-1a) may act to repress RUNX1-mediated transactivation. Interacts with KAT6A and KAT6B. Interacts with SUV39H1, leading to abrogation of transactivating and DNA-binding properties of RUNX1. Interacts with YAP1. Interacts with HIPK2. Interaction with CDK6 prevents myeloid differentiation, reducing its transcription transactivation activity. Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood. 11 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding; Oncoprotein
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; basement membrane; nucleus
Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; calcium ion binding; transcription factor binding; transcription factor activity; ATP binding
Biological Process: transcription from RNA polymerase II promoter; central nervous system development; positive regulation of transcription, DNA-dependent; positive regulation of granulocyte differentiation; regulation of signal transduction; negative regulation of granulocyte differentiation; neuron differentiation; negative regulation of cell proliferation; behavioral response to pain; regulation of transcription, DNA-dependent; myeloid progenitor cell differentiation; regulation of cell differentiation; hemopoiesis; skeletal development; hair follicle morphogenesis; response to retinoic acid; transcription, DNA-dependent; in utero embryonic development; embryonic hemopoiesis; liver development; positive regulation of interferon-gamma production; positive regulation of angiogenesis; regulation of T cell anergy; positive regulation of interleukin-2 production; neuron development; myeloid cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
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Product Notes
The Runx1 runx1 (Catalog #AAA9207083) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Runx1 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Runx1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:2000. Researchers should empirically determine the suitability of the Runx1 runx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Runx1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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