Western Blot (WB)
(Western Blot, non-reducing using T5100-06CX)
Mouse Thrombospondin Monoclonal Antibody | anti-TSP antibody
Thrombospondin (TSP) (BSA & Azide Free) (AP)
Reactivity
Bovine, Canine, Equine, Human, Porcine, Rat, Sheep
Applications
Immunoprecipitation, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
Thrombospondin; Monoclonal Antibody; Thrombospondin (TSP) (BSA & Azide Free) (AP); anti-TSP antibody
Host
Mouse
Reactivity
Bovine, Canine, Equine, Human, Porcine, Rat, Sheep
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3F352
Specificity
Recognizes human Thrombospondin. Shows no crossreactivity with fibronectin, fibrinogen or von Willebrand factor. It blocks TSP-mediated agglutination of fixed RBCs. It shows no effect on TSP-mediated agglutination of fixed, activated platelets. It inhibits both thrombin -and -induced aggregation of washed, live (not fixed) platelets without affecting the secretion of serotonin. Inhibits adhesion of melanoma G361 cells to TSP. Blocks binding of C-terminal domain to Integrin-Associated Protein (IAP)/CD47. Species Crossreactivity: bovine, porcine, equine, canine, sheep, rat.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-TSP antibody
Immunoprecipitation (IP), Western Blot (WB)
Immunogen
Ca2+-replete, native purified human TSP from supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (Non-reduced). Epitope: Platelet- or cell-binding domain at the extreme C-terminus of TSP.
Conjugate
AP
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Western Blot (WB)
(Western Blot, non-reducing using T5100-06CX)
Western Blot (WB)
(Western Blot, non-reducing using T5100-06CX)
Immunoprecipitation (IP)
(Immunoprecipitation, native using T5100-06CX)
Immunoprecipitation (IP)
(Immunoprecipitation, native using T5100-06CX)
Product Categories/Family for anti-TSP antibody
References
1. Dixit VM, et. al. Proc Natl Acad Sci USA 1985, 82:3472-6. 2. Galvin NJ, et. al. J Cell Biol, 1985, 101(4):1434-41. 3. Dixit VM, et. al. Biochemistry, 1985, 24(16):4270-5. 4. Prater CA, et. al. J Cell Biol, 1991, 112(5):1031-40
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Product Notes
The TSP (Catalog #AAA6265931) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Thrombospondin (TSP) (BSA & Azide Free) (AP) reacts with Bovine, Canine, Equine, Human, Porcine, Rat, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Thrombospondin can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the TSP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thrombospondin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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