Western Blot (WB)
(Figure. Western Blot: Sample: Recombinant CALPRO, Human)
Mouse anti-Human Calprotectin (CALPRO) Monoclonal Antibody | anti-CALPRO antibody
Calprotectin (CALPRO) Monoclonal Antibody
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
ELISA: 0.05-2ug/ml
Western Blot (WB)
(Figure. Western Blot: Sample: Recombinant CALPRO, Human)
Western Blot (WB)
(Figure. Western Blot: Sample: Recombinant CALPRO, Human)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
Uniprot Description
S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family.
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: cytoskeleton; cytosol; extracellular region; extracellular space; intermediate filament cytoskeleton; nucleus; plasma membrane
Molecular Function: arachidonic acid binding; calcium ion binding; microtubule binding; protein binding; RAGE receptor binding; zinc ion binding
Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; acute inflammatory response; antimicrobial humoral response; apoptosis; astrocyte development; autophagy; chemokine production; chronic inflammatory response; cytokine production; defense response to bacterium; defense response to fungus; inflammatory response; innate immune response; leukocyte migration involved in inflammatory response; neutrophil chemotaxis; neutrophil degranulation; peptidyl-cysteine S-nitrosylation; positive regulation of cell growth; positive regulation of inflammatory response; positive regulation of NF-kappaB transcription factor activity; positive regulation of peptide secretion; regulation of cytoskeleton organization; response to ethanol; response to lipopolysaccharide; response to zinc ion; sequestering of zinc ion; toll-like receptor signaling pathway; wound healing
Research Articles on CALPRO
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Product Notes
The CALPRO s100a8 (Catalog #AAA2090415) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Calprotectin (CALPRO) Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Calprotectin (CALPRO) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), IHC, Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-5ug/ml Immunocytochemistry in formalin fixed cells: 5-30ug/ml Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml Immunohistochemistry in paraffin section: 5-30ug/ml ELISA: 0.05-2ug/ml. Researchers should empirically determine the suitability of the CALPRO s100a8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calprotectin (CALPRO), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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