Immunohistochemistry (IHC)
(Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit AML1/RUNX1 Polyclonal Antibody | anti-AML1/RUNX1 antibody
Anti-AML1/RUNX1 Antibody (aa242-291)
Predicted: Mouse, Rat
Predicted: Mouse, Rat
IHC-P: 1:50
WB: 1:500-1:1000
Immunohistochemistry (IHC)
(Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (WB)
(Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (WB)
(Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE))
Western Blot (WB)
(Western blot of extracts from Jurkat cells, using AML1 Antibody. The lane on the right was incubated with synthetic peptide.)
Western Blot (WB)
(Western blot of extracts from Jurkat cells, using AML1 Antibody. The lane on the right was incubated with synthetic peptide.)
NCBI and Uniprot Product Information
NCBI Description
Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). Essential for the development of normal hematopoiesis (PubMed:17431401). Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter (PubMed:10207087, PubMed:14970218). Inhibits KAT6B-dependent transcriptional activation (). Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (). Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532). Positively regulates the expression of RORC in T-helper 17 cells ().
Research Articles on AML1/RUNX1
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Product Notes
The AML1/RUNX1 runx1 (Catalog #AAA2401242) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-AML1/RUNX1 Antibody (aa242-291) reacts with Human Predicted: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AML1/RUNX1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ELISA: 1:5000 IHC-P: 1:50 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the AML1/RUNX1 runx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AML1/RUNX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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