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Western Blot (WB) (Western Blot analysis of lysate from 293 cell line (35ug) using MBS643902 (1:1000). A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.)

Rabbit anti-Human RSPO2 Polyclonal Antibody | anti-RSPO2 antibody

RSPO2, CT (RSPO2, R-spondin-2, Roof plate-specific spondin-2) (MaxLight 650)

Gene Names
RSPO2; CRISTIN2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
RSPO2; Polyclonal Antibody; CT (RSPO2; R-spondin-2; Roof plate-specific spondin-2) (MaxLight 650); Roof plate-specific spondin-2; anti-RSPO2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human RSPO2.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Applicable Applications for anti-RSPO2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), FLISA
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 190-218 in the C-terminal region of human RSPO2, UniProt Accession #Q6UXX9; Q8BFU0. Species sequence homology: mouse
Conjugate
MaxLight650
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of lysate from 293 cell line (35ug) using MBS643902 (1:1000). A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.)

Western Blot (WB) (Western Blot analysis of lysate from 293 cell line (35ug) using MBS643902 (1:1000). A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma using MBS643902 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS643902 for immunohistochemistry.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma using MBS643902 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS643902 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis with 293 cells using MBS643902 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow Cytometric analysis with 293 cells using MBS643902 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-RSPO2 antibody
R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling.
Product Categories/Family for anti-RSPO2 antibody
References
1. Kim, K.A., et al. Cell Cycle 5 (1): 23-26 (2006). 2. Kazanskaya, O., et al. Dev. Cell 7 (4):5 25-534 (2004). 3. Clark, H.F., et al. Genome Res. 13(10): 2265-2270 (2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22.0kD
NCBI Official Full Name
R-spondin-2 isoform 1
NCBI Official Synonym Full Names
R-spondin 2
NCBI Official Symbol
RSPO2
NCBI Official Synonym Symbols
CRISTIN2
NCBI Protein Information
R-spondin-2; R-spondin 2 homolog; roof plate-specific spondin-2
UniProt Protein Name
R-spondin-2
Protein Family
UniProt Gene Name
RSPO2
UniProt Synonym Gene Names
hRspo2
UniProt Entry Name
RSPO2_HUMAN

NCBI Description

R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling (Kim et al., 2006 [PubMed 16357527]).[supplied by OMIM, Mar 2008]

Uniprot Description

R-spondin-2: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cell development/differentiation; Secreted

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: heparin binding; G-protein-coupled receptor binding; receptor binding

Biological Process: embryonic forelimb morphogenesis; osteoblast differentiation; Wnt receptor signaling pathway; embryonic hindlimb morphogenesis; bone mineralization

Research Articles on RSPO2

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Product Notes

The RSPO2 rspo2 (Catalog #AAA6344224) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RSPO2, CT (RSPO2, R-spondin-2, Roof plate-specific spondin-2) (MaxLight 650) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RSPO2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RSPO2 rspo2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RSPO2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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