Rabbit anti-Human DUSP8 / HB5 Polyclonal Antibody | anti-DUSP8 antibody

Rabbit Polyclonal to Human DUSP8 / HB5

Cat. Num. AAA243223

• Gene Names: DUSP8; HB5; HVH8; HVH-5; C11orf81
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Ammonium sulfate precipitation
• Synonyms: DUSP8 / HB5; Polyclonal Antibody; Rabbit Polyclonal to Human DUSP8 / HB5; Anti-DUSP8 / HB5 Antibody (C-Terminus) IHC-plus; DUSP8; C11orf81; Dusp 8; Dual specificity phosphatase 8; HB5; HVH-5; Hvh5; HVH8; M3/6; VH5; Human DUSP8; anti-DUSP8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Ammonium sulfate precipitation
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 625

• Applicable Applications for anti-DUSP8 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1:1000)
• Target Species: Human
• Immunogen Description: KLH conjugated synthetic peptide selected from the C-terminal region of human DUSP8.
• Immunogen Type: Synthetic peptide
• Immunogen: DUSP8 / HB5 antibody was raised against kLH conjugated synthetic peptide selected from the C-terminal region of human DUSP8.
• Antigen Modification: C-Terminus
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-DUSP8 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-DUSP8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot of anti-DUSP8 Pab in HeLa cell line lysate (35 ug/lane). DUSP8(arrow) was detected using the purified Pab (1:50 dilution).)

Related Product Information for anti-DUSP8 antibody

DUSP8 encodes a dual specificity MAP kinase phosphatase which has been shown to hydrolyze p-nitrophenylphosphate and inactivate mitogen-activated protein kinases in vitro. The mouse homologue, M3/6, has been demonstrated to be uniquely specific for JNK and p38 kinases.

Product Categories/Family for anti-DUSP8 antibody

Subfamily: Dual specificity MKP

NCBI and Uniprot Product Information

• NCBI GI #: 153281158
• NCBI GeneID: 1850
• NCBI Accession #: NP_004411.2
• NCBI GenBank Nucleotide #: NM_004420.2
• UniProt Accession #: Q13202; Q86SS8
• Molecular Weight: 65,827 Da
• NCBI Official Full Name: dual specificity protein phosphatase 8
• NCBI Official Synonym Full Names: dual specificity phosphatase 8
• NCBI Official Symbol: DUSP8
• NCBI Official Synonym Symbols: HB5; HVH8; HVH-5; C11orf81
• NCBI Protein Information: dual specificity protein phosphatase 8; H1 phosphatase, vaccinia virus homolog; dual specificity protein phosphatase hVH-5; serine/threonine specific protein phosphatase
• UniProt Protein Name: Dual specificity protein phosphatase 8
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP8
• UniProt Synonym Gene Names: C11orf81; VH5
• UniProt Entry Name: DUS8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2. [provided by RefSeq, Jul 2008]

Uniprot Description

DUSP8: a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (ERK, JNK, p38). Inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin.

Protein type: EC 3.1.3.16; Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasm; nucleus

Molecular Function: protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: protein amino acid dephosphorylation; inactivation of MAPK activity