Western Blot (WB)
(Western BlotPositive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysateAll lanes: RPIA antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 34 kDaObserved band size: 33 kDa)
Rabbit anti-Human RPIA Polyclonal Antibody | anti-RPIA antibody
RPIA Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
IHC: 1:200-1:500
Western Blot (WB)
(Western BlotPositive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysateAll lanes: RPIA antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 34 kDaObserved band size: 33 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysateAll lanes: RPIA antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 34 kDaObserved band size: 33 kDa)
Immunohistochemistry (IHC)
(IHC image of MBS7135042 diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
Immunohistochemistry (IHC)
(IHC image of MBS7135042 diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an enzyme, which catalyzes the reversible conversion between ribose-5-phosphate and ribulose-5-phosphate in the pentose-phosphate pathway. This gene is highly conserved in most organisms. The enzyme plays an essential role in the carbohydrate metabolism. Mutations in this gene cause ribose 5-phosphate isomerase deficiency. A pseudogene is found on chromosome 18. [provided by RefSeq, Mar 2010]
Uniprot Description
RPIA: Defects in RPIA are the cause of ribose 5-phosphate isomerase deficiency (RPID). A patient has been described with a deficiency of ribose 5-phosphate isomerase who presented with leukoencephalopathy and peripheral neuropathy. Proton magnetic resonance spectroscopy of the brain revealed a highly elevated level of the polyols ribitol and D-arabitol, which were subsequently also found in high concentrations in body fluids. Deficient activity of RPIA, one of the pentose phosphate pathway enzymes, has been demonstrated in fibroblasts. Belongs to the ribose 5-phosphate isomerase family.
Protein type: EC 5.3.1.6; Carbohydrate Metabolism - pentose phosphate pathway; Isomerase
Chromosomal Location of Human Ortholog: 2p11.2
Cellular Component: intracellular membrane-bound organelle; cytosol
Molecular Function: monosaccharide binding; ribose-5-phosphate isomerase activity
Biological Process: pentose-phosphate shunt; carbohydrate metabolic process; pentose-phosphate shunt, non-oxidative branch; pathogenesis
Disease: Ribose 5-phosphate Isomerase Deficiency
Research Articles on RPIA
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Product Notes
The RPIA rpia (Catalog #AAA7135042) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPIA Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPIA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000-1:5000 IHC: 1:200-1:500. Researchers should empirically determine the suitability of the RPIA rpia for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPIA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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