Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ABCG1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit ABCG1 Polyclonal Antibody | anti-ABCG1 antibody
ABCG1 Polyclonal Antibody
IHC: 1:50 - 1:200
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ABCG1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ABCG1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
ABCG1: Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Transporter, ABC family; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: nucleoplasm; Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; recycling endosome; intracellular membrane-bound organelle; mitochondrion; integral to plasma membrane; plasma membrane; endosome; external side of plasma membrane
Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; phospholipid transporter activity; protein heterodimerization activity; cholesterol transporter activity; cholesterol binding; toxin transporter activity; phospholipid binding; sterol-transporting ATPase activity; ADP binding; ATP binding
Biological Process: cholesterol metabolic process; detection of hormone stimulus; response to high density lipoprotein stimulus; positive regulation of cholesterol biosynthetic process; cholesterol efflux; lipoprotein metabolic process; intracellular cholesterol transport; response to lipid; response to organic substance; cholesterol homeostasis; amyloid precursor protein catabolic process; reverse cholesterol transport; phospholipid efflux; regulation of transcription, DNA-dependent; transmembrane transport; phospholipid homeostasis
Research Articles on ABCG1
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Product Notes
The ABCG1 abcg1 (Catalog #AAA129616) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ABCG1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ABCG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the ABCG1 abcg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ABCG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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