Immunofluorescence (IF)
(Immunofluorescence staining of Human VEGFR1 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human VEGFR1 monoclonal antibody (dilution ratio 1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody. Positive staining was localized to plasma membrane.)
Mouse anti-Human FLT1 Monoclonal Antibody | anti-FLT1 antibody
Anti-FLT1 Antibody, Mouse Monoclonal
No cross-reactivity in ELISA with Human VEGFR2, Human VEGFR3293 cell lysate
ICC/IF: 1:20-1:100
Immunofluorescence (IF)
(Immunofluorescence staining of Human VEGFR1 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human VEGFR1 monoclonal antibody (dilution ratio 1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody. Positive staining was localized to plasma membrane.)
Immunofluorescence (IF)
(Immunofluorescence staining of Human VEGFR1 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human VEGFR1 monoclonal antibody (dilution ratio 1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody. Positive staining was localized to plasma membrane.)
Kendall R.L., et al., (1993), Inhibition of vascular endothelial cell growth factor activity by an endogenously encoded soluble receptor.Proc. Natl. Acad. Sci. U.S.A. 90:10705-10709.
Herley M.T., et al., (1999), Characterization of the VEGF binding site on the Flt-1 receptor.Biochem. Biophys. Res. Commun. 262:731-738.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.[provided by RefSeq, May 2009]
Uniprot Description
VEGFR1: a receptor tyrosine kinase of the VEGFR family. Receptor for VEGF, VEGFB and PGF. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Two splice variant isoforms have been described. Isoform SFlt1 may have an inhibitory role in angiogenesis.
Protein type: Protein kinase, TK; Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; VEGFR family
Chromosomal Location of Human Ortholog: 13q12
Cellular Component: extracellular space; focal adhesion; integral to plasma membrane; plasma membrane; receptor complex; endosome
Molecular Function: vascular endothelial growth factor receptor activity; identical protein binding; protein binding; growth factor binding; transmembrane receptor protein tyrosine kinase activity; ATP binding
Biological Process: cell migration; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of vascular endothelial growth factor receptor signaling pathway; patterning of blood vessels; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of MAP kinase activity; monocyte chemotaxis; positive regulation of angiogenesis; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; blood vessel morphogenesis; sprouting angiogenesis; embryonic morphogenesis; cell differentiation; vascular endothelial growth factor receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration
Research Articles on FLT1
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Product Notes
The FLT1 flt1 (Catalog #AAA8101209) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-FLT1 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FLT1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF). ELISA: 1:1000-1:2000 ICC/IF: 1:20-1:100. Researchers should empirically determine the suitability of the FLT1 flt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FLT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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