Rabbit anti-Human RPAIN Polyclonal Antibody | anti-RPAIN antibody

RPAIN, NT (RPAIN, RIP, RPA-interacting protein) (Biotin)

Cat. Num. AAA6343206

• Gene Names: RPAIN; RIP; HRIP
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPAIN; Polyclonal Antibody; NT (RPAIN; RIP; RPA-interacting protein) (Biotin); RPA-interacting protein; anti-RPAIN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 231

• Applicable Applications for anti-RPAIN antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPAIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of human RPAIN.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPAIN Antibody (N-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RPAIN antibody detected the RPAIN protein (arrow).)

Related Product Information for anti-RPAIN antibody

RPAIN mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. Isoform 2 is sumoylated and mediates the localization of RPA complex into the PML body of the nucleus, thereby participating in RPA function in DNA metabolism.

Product Categories/Family for anti-RPAIN antibody

Antibodies; DNA Replication; Repair

References

Davila, S., et al. Genes Immun. 11(3):232-238(2010). Dagle, J.M., et al. Pediatrics 123(4):1116-1123(2009). Lamesch, P., et al. Genomics 89(3):307-315(2007). Park, J., et al. Mol. Cell. Biol. 25(18):8202-8214(2005). Chen, J.Z., et al. DNA Cell Biol. 24(7):464-469(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 237858698
• NCBI GeneID: 84268
• NCBI Accession #: NP_001153715.1; NP_001028174.2
• NCBI GenBank Nucleotide #: NP_001153715.1
• UniProt Accession #: Q86UA6
• NCBI Official Full Name: RPA-interacting protein isoform a
• NCBI Official Synonym Full Names: RPA interacting protein
• NCBI Official Symbol: RPAIN
• NCBI Official Synonym Symbols: RIP; HRIP
• NCBI Protein Information: RPA-interacting protein
• UniProt Protein Name: RPA-interacting protein
• Protein Family: RPA-interacting protein
• UniProt Gene Name: RPAIN
• UniProt Synonym Gene Names: RIP; hRIP
• UniProt Entry Name: RIP_HUMAN

Uniprot Description

RPAIN: Mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. Isoform 2 is sumoylated and mediates the localization of RPA complex into the PML body of the nucleus, thereby participating in RPA function in DNA metabolism. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; DNA replication

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: PML body; cytoplasm; nucleolus; nucleus

Molecular Function: metal ion binding; protein complex binding

Biological Process: protein import into nucleus; DNA-dependent DNA replication; DNA repair; response to UV; DNA recombination