Western Blot (WB)
(RPAIN Antibody (N-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RPAIN antibody detected the RPAIN protein (arrow).)
Rabbit anti-Human RPAIN Polyclonal Antibody | anti-RPAIN antibody
RPAIN, NT (RPAIN, RIP, RPA-interacting protein)
Gene Names
RPAIN; RIP; HRIP
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
RPAIN; Polyclonal Antibody; NT (RPAIN; RIP; RPA-interacting protein); Anti -RPAIN; anti-RPAIN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-RPAIN antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
RPAIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of human RPAIN.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(RPAIN Antibody (N-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RPAIN antibody detected the RPAIN protein (arrow).)
Western Blot (WB)
(RPAIN Antibody (N-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RPAIN antibody detected the RPAIN protein (arrow).)
Related Product Information for anti-RPAIN antibody
RPAIN mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. Isoform 2 is sumoylated and mediates the localization of RPA complex into the PML body of the nucleus, thereby participating in RPA function in DNA metabolism.
Product Categories/Family for anti-RPAIN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
24,784 Da
NCBI Official Full Name
RPAIN protein
NCBI Official Synonym Full Names
RPA interacting protein
NCBI Official Symbol
RPAIN
NCBI Official Synonym Symbols
RIP; HRIP
NCBI Protein Information
RPA-interacting protein; nuclear transporter; RAP interaction protein
UniProt Protein Name
RPA-interacting protein
Protein Family
UniProt Gene Name
RPAIN
UniProt Synonym Gene Names
RIP; hRIP
UniProt Entry Name
RIP_HUMAN
Uniprot Description
RPAIN: Mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. Isoform 2 is sumoylated and mediates the localization of RPA complex into the PML body of the nucleus, thereby participating in RPA function in DNA metabolism. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA repair, damage; DNA replication
Chromosomal Location of Human Ortholog: 17p13.2
Cellular Component: PML body; cytoplasm; nucleolus; nucleus
Molecular Function: metal ion binding; protein complex binding
Biological Process: protein import into nucleus; DNA repair; DNA-dependent DNA replication; response to UV; DNA recombination
Research Articles on RPAIN
Similar Products
Product Notes
The RPAIN rpain (Catalog #AAA6003696) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPAIN, NT (RPAIN, RIP, RPA-interacting protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPAIN can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the RPAIN rpain for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPAIN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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