Rabbit anti-Human RPA43 Polyclonal Antibody | anti-RPA43 antibody

RPA43, CT (TWISTNB, DNA-directed RNA polymerase I subunit RPA43, Twist neighbor protein)

Cat. Num. AAA648826

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RPA43; Polyclonal Antibody; CT (TWISTNB; DNA-directed RNA polymerase I subunit RPA43; Twist neighbor protein); Anti -RPA43; anti-RPA43 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-RPA43 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: RPA43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306-336 amino acids from the C-terminal region of human RPA43.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RPA43 Antibody (C-term) in Jurkat,K562 cell line lysates (35ug/lane).RPA43 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(RPA43 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPA43 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-RPA43 antibody

RPA43 is DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through its association with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters.

Product Categories/Family for anti-RPA43 antibody

Antibodies; Abs to DNA Replication; Repair

NCBI and Uniprot Product Information

• NCBI GI #: 6324916
• NCBI GeneID: 854518
• NCBI Accession #: NP_014985.1
• NCBI GenBank Nucleotide #: NM_001183760.1
• UniProt Accession #: P46669; Q99187; D6W335
• Molecular Weight: 36,224 Da
• NCBI Official Full Name: Rpa43p
• NCBI Official Symbol: RPA43
• NCBI Protein Information: Rpa43p
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA43
• Protein Family: DNA-directed RNA polymerase
• UniProt Gene Name: RPA43
• UniProt Synonym Gene Names: RRN12; A43
• UniProt Entry Name: RPA43_YEAST

Uniprot Description

Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through its association with RRN3 is involved in recruitment of Pol I to rDNA promoters. In vitro, the A13-A43 subcomplex binds single-stranded RNA. Ref.5 Ref.15

Subunit structure: Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits. Part of a Pol I subcomplex consisting of the subunits A14 and A43. Interacts with RPA14, RPO26/ABC23 and RRN3. Ref.5 Ref.6 Ref.12

Subcellular location: Nucleus › nucleolus Ref.13.

Post-translational modification: Contains an average of four phosphates per molecule.

Miscellaneous: Present with 4070 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the eukaryotic RPA43 RNA polymerase subunit family.