Rabbit M-RIP Polyclonal Antibody | anti-M-RIP antibody

M-RIP polyclonal antibody

Cat. Num. AAA3014608

• Gene Names: MPRIP; MRIP; RIP3; M-RIP; RHOIP3; p116Rip
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
• Synonyms: M-RIP; Polyclonal Antibody; M-RIP polyclonal antibody; Myosin phosphatase Rho-interacting protein; Rho-interacting protein 3; RIP3; p116Rip; MPRIP; anti-M-RIP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: M-RIP polyclonal antibody detects endogenous levels of M-RIP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Applicable Applications for anti-M-RIP antibody: Western Blot (WB)
• Application Notes: WB: 1:5000~1:10000
• Immunogen: Synthetic peptide, corresponding to Human M-RIP.
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of M-RIP polyclonal antibody at 1:5000 dilutionLane1:PC12 whole cell lysate(40ug)Lane2:CT-26 whole cell lysate(40ug)Lane3:H1792 whole cell lysate(40ug)Lane4:Myla2059 whole cell lysate(40ug)Lane5:PC3 whole cell lysate(40ug)Lane6:SGC7901 whole cell lysate(40ug))

Related Product Information for anti-M-RIP antibody

Myosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics. M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA. Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility. M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho/ROCK, and actin. Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics.

NCBI and Uniprot Product Information

• NCBI GI #: 50980307
• NCBI GeneID: 23164
• NCBI Accession #: NP_055949.2
• NCBI GenBank Nucleotide #: NM_015134.3
• UniProt Accession #: Q6WCQ1; Q3KQZ5; Q5FB94; Q6DHW2; Q7Z5Y2; Q8N390; Q96G40
• Molecular Weight: 116,533 Da
• NCBI Official Full Name: myosin phosphatase Rho-interacting protein isoform 1
• NCBI Official Synonym Full Names: myosin phosphatase Rho interacting protein
• NCBI Official Symbol: MPRIP
• NCBI Official Synonym Symbols: MRIP; RIP3; M-RIP; RHOIP3; p116Rip
• NCBI Protein Information: myosin phosphatase Rho-interacting protein; Rho interacting protein 3
• UniProt Protein Name: Myosin phosphatase Rho-interacting protein
• UniProt Gene Name: MPRIP
• UniProt Synonym Gene Names: KIAA0864; MRIP; RHOIP3; M-RIP; RIP3
• UniProt Entry Name: MPRIP_HUMAN

Uniprot Description

RIP3: Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F- actin-binding region leads to disassembly of stress fibers in neuronal cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, Rac/Rho; GAPs

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: focal adhesion; cytoplasm; actin cytoskeleton

Molecular Function: protein binding; actin binding