Western Blot (WB)
(Anti-PPIL3 rabbit polyclonal antibody at 1:500 dilution Lane A: 293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:18 kDa)
Rabbit anti-Human PPIL3 Polyclonal Antibody | anti-PPIL3 antibody
Anti-PPIL3 Antibody, Rabbit Polyclonal
Gene Names
PPIL3; CYPJ
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PPIL3; Polyclonal Antibody; Anti-PPIL3 Antibody; Rabbit Polyclonal; PPIL3 Antibody; Rabbit PAb; Antigen Affinity Purified; peptidylprolyl isomerase (cyclophilin)-like 3; Anti-CYPJ Antibody; anti-PPIL3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PPIL3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PPIL3 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthetic peptide corresponding to the center region of the Human PPIL3
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PPIL3, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PPIL3 rabbit polyclonal antibody at 1:500 dilution Lane A: 293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:18 kDa)
Western Blot (WB)
(Anti-PPIL3 rabbit polyclonal antibody at 1:500 dilution Lane A: 293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:18 kDa)
Related Product Information for anti-PPIL3 antibody
PPIL3 (Peptidylprolyl Isomerase Like 3) is a Protein Coding gene. The PPIL3 gene, located on 2q33.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, M.oryzae, N.crassa, A.thaliana, rice, and frog. The PPIL3 gene consisted of eight exons spanning more than 18 kb of genomic DNA. RT-PCR analysis indicated that PPIL3 was ubiquitously expressed in adult human tissues. This gene encodes a member of the cyclophilin family. Cyclophilins catalyze the cis-trans isomerization of peptidylprolyl imide bonds in oligopeptides. They have been proposed to act either as catalysts or as molecular chaperones in protein-folding events. 239 organisms have orthologs with the human gene PPIL3.
References
Zhou Z, et al. (2001) Molecular cloning and characterization of a novel peptidylprolyl isomerase (cyclophilin)-like gene (ppil3) from human fetal brain. Cytogenet Cell Genet 92 (3-4): 231-236.Gong Z, et al. (2017) Expression and significance of cyclophilin j in primary gastric adenocarcinoma. Anticancer Res 37 (8): 4475-4481.Chen J, et al. (2015) Cyclophilin j is a novel peptidyl-prolyl isomerase and target for repressing the growth of hepatocellular carcinoma. PLoS One 10 (5): e0127668.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,627 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase-like 3 isoform PPIL3a
NCBI Official Synonym Full Names
peptidylprolyl isomerase (cyclophilin)-like 3
NCBI Official Symbol
PPIL3
NCBI Official Synonym Symbols
CYPJ
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase-like 3; PPIase; PPIase-like protein 3; cyclophilin J; cyclophilin-like protein 3; cyclophilin-like protein PPIL3; peptidylprolyl cis-trans isomerase-like protein 3; rotamase PPIL3
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase-like 3
Protein Family
UniProt Gene Name
PPIL3
UniProt Synonym Gene Names
PPIase; CyPJ
UniProt Entry Name
PPIL3_HUMAN
NCBI Description
This gene encodes a member of the cyclophilin family. Cyclophilins catalyze the cis-trans isomerization of peptidylprolyl imide bonds in oligopeptides. They have been proposed to act either as catalysts or as molecular chaperones in protein-folding events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2008]
Uniprot Description
PPIL3: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing. Belongs to the cyclophilin-type PPIase family. PPIL3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cyclophilin; EC 5.2.1.8; Isomerase; Spliceosome
Chromosomal Location of Human Ortholog: 2q33.1
Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity
Biological Process: nuclear mRNA splicing, via spliceosome; protein peptidyl-prolyl isomerization; protein folding
Research Articles on PPIL3
Similar Products
Product Notes
The PPIL3 ppil3 (Catalog #AAA8104008) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PPIL3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPIL3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PPIL3 ppil3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPIL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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