Rabbit anti-Human, Mouse RNF8 Polyclonal Antibody | anti-RNF8 antibody

RNF8, CT (RNF8, KIAA0646, E3 ubiquitin-protein ligase RNF8, RING finger protein 8) (APC)

Cat. Num. AAA6342985

• Gene Names: RNF8; hRNF8
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RNF8; Polyclonal Antibody; CT (RNF8; KIAA0646; E3 ubiquitin-protein ligase RNF8; RING finger protein 8) (APC); RING finger protein 8; anti-RNF8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-RNF8 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RNF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 334-364 amino acids from the C-terminal region of human RNF8.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RNF8 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RNF8 antibody detected the RNF8 protein (arrow).)

Western Blot (WB)

(RNF8 Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the RNF8 antibody detected the RNF8 protein (arrow).)

Related Product Information for anti-RNF8 antibody

The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Product Categories/Family for anti-RNF8 antibody

Antibodies; Ubiquitin Ligases

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Koike, A., et al. Cancer Res. 70(17):6746-6756(2010). Lilley, C.E., et al. EMBO J. 29(5):943-955(2010). Noon, A.T., et al. Nat. Cell Biol. 12(2):177-184(2010). Ramachandran, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(2):809-814(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4504867
• NCBI GeneID: 9025
• NCBI Accession #: NP_003949.1; NP_898901.1
• NCBI GenBank Nucleotide #: NM_003958.3
• UniProt Accession #: O76064; Q53H16; Q5NKW5; A6NN24; A8MYC0; B4DPG0
• Molecular Weight: 50,829 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF8 isoform 1
• NCBI Official Synonym Full Names: ring finger protein 8
• NCBI Official Symbol: RNF8
• NCBI Official Synonym Symbols: hRNF8
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF8
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF8
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF8
• UniProt Synonym Gene Names: KIAA0646; hRNF8
• UniProt Entry Name: RNF8_HUMAN

NCBI Description

The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

RNF8: E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'- linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Also controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites. Also recruited at DNA interstrand cross-links (ICLs) sites and catalyzes 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Promotes the formation of 'Lys-63'-linked polyubiquitin chains via interactions with the specific ubiquitin-conjugating UBE2N/UBC13 and ubiquitinates non-histone substrates such as PCNA. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Also catalyzes the formation of 'Lys-48'-linked polyubiquitin chains via interaction with the ubiquitin- conjugating UBE2L6/UBCH8, leading to degradation of substrate proteins such as CHEK2, JMJD2A/KDM4A and KU80/XRCC5: it is still unclear how the preference toward 'Lys-48'- versus 'Lys-63'-linked ubiquitination is regulated but it could be due to RNF8 ability to interact with specific E2 specific ligases. For instance, interaction with phosphorylated HERC2 promotes the association between RNF8 and UBE2N/UBC13 and favors the specific formation of 'Lys-63'-linked ubiquitin chains. Promotes non-homologous end joining (NHEJ) by promoting the 'Lys-48'-linked ubiquitination and degradation the of KU80/XRCC5. Following DNA damage, mediates the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF168, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. In addition to its function in damage signaling, also plays a role in higher-order chromatin structure by mediating extensive chromatin decondensation. Involved in the activation of ATM by promoting histone H2B ubiquitination, which indirectly triggers histone H4 'Lys-16' acetylation (H4K16ac), establishing a chromatin environment that promotes efficient activation of ATM kinase. Required in the testis, where it plays a role in the replacement of histones during spermatogenesis. At uncapped telomeres, promotes the joining of deprotected chromosome ends by inducing H2A ubiquitination and TP53BP1 recruitment, suggesting that it may enhance cancer development by aggraving telomere-induced genome instability in case of telomeric crisis. Promotes the assembly of RAD51 at DNA DSBs in the absence of BRCA1 and TP53BP1 Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2. Belongs to the RNF8 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Histone-binding; Transcription, coactivator/corepressor; EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system; DNA repair, damage; Ligase

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: chromosome, telomeric region; nucleolus; midbody; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; protein homodimerization activity; acid-amino acid ligase activity; histone binding; zinc ion binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; chromatin binding

Biological Process: ubiquitin-dependent protein catabolic process; mitosis; protein autoubiquitination; positive regulation of DNA repair; histone H2B ubiquitination; isotype switching; histone exchange; double-strand break repair via nonhomologous end joining; cell division; double-strand break repair; response to ionizing radiation; response to DNA damage stimulus; spermatid development; histone H2A ubiquitination