Mouse FKBP52 Monoclonal Antibody | anti-FKBP4 antibody

FKBP52 Antibody

Cat. Num. AAA801494

• Gene Names: FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
• Reactivity: Canine, Hamster, Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: FKBP52; Monoclonal Antibody; FKBP52 Antibody; FK506 binding protein 4; FKBP4; FKBP59; HBI; Hsp56; p52; p59; PPIase; Rotamase; T cell FK506 binding protein; anti-FKBP4 antibody

• Host: Mouse
• Reactivity: Canine, Hamster, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: Hi52C
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 459

• Applicable Applications for anti-FKBP4 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC: Paraffin embedded)
• Application Notes: 1:2000 (WB), 5ug with 10-20uL Protein A beads (IP), 1:250 (IHC)
• Certificate of Analysis: 0.5ug/ml was sufficient for detection of FKBP52 in 20ug total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Tissue: prostate tissue (ductal epithelial cells). Species: Human. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1:1000. Courtesy of: David F. Smith, Mayo Clinic, USA.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)

Western Blot (WB)

(Western Blot analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1.5 ug/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Tissue: MCF-7 cells (metastatic mammary gland/breast cell line). Species: Human. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Courtesy of: Tom Ratajczak, Univ. of W. Australia.)

Western Blot (WB)

(Western Blot analysis of Human Cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1.5 ug/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Related Product Information for anti-FKBP4 antibody

Background Info: Detects an ~52kDa protein representing FKBP52 in cell lysate. Also detects FKBP52 in whole tissue extracts from rat kidney and rat and mouse testes. Heavy chain migrates close to FKBP52 on SDS PAGE.

Scientific Background: Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).

Product Categories/Family for anti-FKBP4 antibody

Chaperones; Heat Shock; Trafficking

References

1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F. (2003) J. Biol. Chem. 278(19): 17388-17394. 2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7): 4597-4600. 3. Wu, B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101(22): 8348-8353. 4. Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146(7):3194-3201. 5. Cox M.B. et al. (2007) Molecular Endocrinology. Epub.

NCBI and Uniprot Product Information

• NCBI GI #: 4503729
• NCBI GeneID: 2288
• NCBI Accession #: NP_002005.1
• NCBI GenBank Nucleotide #: NM_002014.3
• UniProt Accession #: Q02790; Q9UCP1; Q9UCV7; D3DUQ1
• Molecular Weight: 51,805 Da
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase FKBP4
• NCBI Official Synonym Full Names: FK506 binding protein 4, 59kDa
• NCBI Official Symbol: FKBP4
• NCBI Official Synonym Symbols: HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP4; rotamase; HSP binding immunophilin; 51 kDa FK506-binding protein; FK506-binding protein 4 (59kD); T-cell FK506-binding protein, 59kD; peptidylprolyl cis-trans isomerase
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP4
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: FKBP4
• UniProt Synonym Gene Names: FKBP52; PPIase FKBP4; FKBP51; 52 kDa FKBP; FKBP-52; p59; FKBP-4; HBI
• UniProt Entry Name: FKBP4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

FKBP4: Immunophilin protein with PPIase and co-chaperone activities. Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.

Protein type: Chaperone; Isomerase; EC 5.2.1.8

Chromosomal Location of Human Ortholog: 12p13.33

Cellular Component: nucleoplasm; microtubule; endoplasmic reticulum membrane; mitochondrion; cell soma; perinuclear region of cytoplasm; cytoplasm; cytosol

Molecular Function: protein binding, bridging; protein binding; peptidyl-prolyl cis-trans isomerase activity; FK506 binding; GTP binding; heat shock protein binding; phosphoprotein binding; tau protein binding; ATP binding; copper-dependent protein binding; glucocorticoid receptor binding

Biological Process: prostate gland development; male sex differentiation; steroid hormone receptor complex assembly; protein complex localization; protein peptidyl-prolyl isomerization; protein folding; negative regulation of microtubule polymerization; negative regulation of microtubule polymerization or depolymerization; androgen receptor signaling pathway; copper ion transport; embryo implantation