Rabbit anti-Mouse Rnasel Polyclonal Antibody | anti-Rnasel antibody

Rnasel, NT (Rnasel, Rns4, 2-5A-dependent ribonuclease, Ribonuclease 4, Ribonuclease L) (Azide free) (HRP)

Cat. Num. AAA6342482

• Gene Names: Rnasel; E230029I04Rik
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Rnasel; Polyclonal Antibody; NT (Rnasel; Rns4; 2-5A-dependent ribonuclease; Ribonuclease 4; Ribonuclease L) (Azide free) (HRP); Ribonuclease L; anti-Rnasel antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Rnasel antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Rnasel antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-167 amino acids from the N-terminal region of Mouse Rnasel.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Rnasel Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Mouse Rnasel antibody detected the Mouse Rnasel protein (arrow).)

Western Blot (WB)

(Mouse Rnasel Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Rnasel antibody detected the Rnasel protein (arrow).)

Related Product Information for anti-Rnasel antibody

Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. Might play a central role in the regulation of mRNA turnover (By similarity).

Product Categories/Family for anti-Rnasel antibody

Antibodies; Enzymes; Ribonuclease

References

Ireland, D.D., et al. PLoS Pathog. 5 (10), E1000602 (2009): Andersen, J.B., et al. RNA Biol 6(3):305-315(2009). Salehzada, T., et al. PLoS ONE 4 (10), E7563 (2009): Li, X.L., et al. Proc. Natl. Acad. Sci. U.S.A. 105(52):20816-20821(2008). Malathi, K., et al. Nature 448(7155):816-819(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 31982957
• NCBI GeneID: 24014
• NCBI Accession #: NP_036012.1
• NCBI GenBank Nucleotide #: NM_011882.2
• UniProt Accession #: Q05921; Q9ERU7
• Molecular Weight: 83,275 Da
• NCBI Official Full Name: 2-5A-dependent ribonuclease
• NCBI Official Synonym Full Names: ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent)
• NCBI Official Symbol: Rnasel
• NCBI Official Synonym Symbols: E230029I04Rik
• NCBI Protein Information: 2-5A-dependent ribonuclease
• UniProt Protein Name: 2-5A-dependent ribonuclease
• Protein Family: 2-5A-dependent ribonuclease
• UniProt Gene Name: Rnasel
• UniProt Synonym Gene Names: Rns4; 2-5A-dependent RNase; RNase L

Uniprot Description

Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis. Might play a central role in the regulation of mRNA turnover (). Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length ().